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§专利与标准 2 篇

737License CRISPR patents for free to share gene editing globally
站内全文Nature · 2021 · 10.1038/d41586-021-02420-x
站内全文 PDF63 KBPubMed 34493846杂志页 DOI
1171Waive CRISPR patents to meet food needs in low-income countries
站内全文Nature · 2021 · 10.1038/d41586-021-02397-7
站内全文 PDF57 KBPubMed 34489579杂志页 DOI

§一步法与一管化体系 69 篇

11Activation mechanism and integrated one-pot assay of chiral-like cRNA-enhanced CRISPR/Cas12a systems
站内全文Nature Communications · 2026 · 10.1038/s41467-026-71675-7
站内全文 PDF107.5 MBPubMed 41951661杂志页 DOI
13Advanced One-Pot RPA-CRISPR/Cas12a Reaction with Glycerol and Betaine for High-Sensitivity Diagnosis of mecA -Carrying Strains in Clinical Samples
站内全文OA 免费ACS Omega · 2025 · 10.1021/acsomega.4c09078
站内全文 PDF2.7 MB出版方 OA 全文ACS OmegaPubMed 39959114杂志页 DOI
21All-in-One Dual CRISPR-Cas12a (AIOD-CRISPR) Assay: A Case for Rapid, Ultrasensitive and Visual Detection of Novel Coronavirus SARS-CoV-2 and HIV virus
站内全文OA 免费Nature Communications · 2020 · 10.1101/2020.03.19.998724
站内全文 PDF3.1 MB出版方 OA 全文Nature CommunicationsPubMed 32511323杂志页 DOI
28Amplification-free DNA Sensor for the One-Step Detection of the Hepatitis B Virus Using an Automated Paper-Based Lateral Flow Electrochemical Device
站内全文Analytical Chemistry · 2020 · 10.1021/acs.analchem.0c04283
站内全文 PDF3.9 MBPubMed 33326737杂志页 DOI
50An extraction-free and one-pot two-temperature CRISPR/Cas12b system for visual detection of Group B Streptococcus by integrating with RPA
站内全文Journal of Clinical Microbiology · 2025 · 10.1128/jcm.00819-25
站内全文 PDF1.5 MBPubMed 40970715杂志页 DOI
102A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome
站内全文OA 免费Scientific Reports · 2016 · 10.1038/srep37895
站内全文 PDF1.1 MB出版方 OA 全文Scientific ReportsPubMed 27883076杂志页 DOI
115A dual‐probe one‐pot RPA ‐ CRISPR /Cas12a: a highly sensitive and rapid method for detection of Phytophthora vignae
站内全文OA 免费Pest Management Science · 2025 · 10.1002/ps.8932
站内全文 PDF3.3 MB出版方 OA 全文Pest Management SciencePubMed 40417776杂志页 DOI
139A novel cloning strategy for one-step assembly of multiplex CRISPR vectors
站内全文OA 免费Scientific Reports · 2018 · 10.1038/s41598-018-35727-3
站内全文 PDF1.3 MB出版方 OA 全文Scientific ReportsPubMed 30504793杂志页 DOI
145A novel one-tube RPA/CRISPR melting curve detection sensing system based on unique 3′-toehold nucleic acid aptamer for Bacillus anthracis detection
站内全文Talanta · 2025 · 10.1016/j.talanta.2025.128306
站内全文 PDF7.1 MBPubMed 40398044杂志页 DOI
149A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs
站内全文OA 免费Nature Biomedical Engineering · 2023 · 10.1038/s41551-023-01033-1
站内全文 PDF3.5 MB出版方 OA 全文Nature Biomedical EngineeringPubMed 37106152杂志页 DOI
150A one-pot method for universal Dengue virus detection by combining RT-RPA amplification and CRISPR/Cas12a assay
站内全文BMC Microbiology · 2025 · 10.21203/rs.3.rs-5729352/v1
站内全文 PDF2.2 MBPubMed 40128655杂志页 DOI
152A One-Pot Reverse Transcriptase-Mediated, Pre-amplification-Free CRISPR/Cas12a Assay for Ultrasensitive Nucleic Acid Detection
站内全文ACS Sensors · 2026 · 10.1021/acssensors.6c01202
站内全文 PDF6.4 MBPubMed 42603302杂志页 DOI
153A one-tube autocatalytic transcription-driven CRISPR cascade for ultrasensitive mRNA detection
站内全文Biosensors and Bioelectronics · 2026 · 10.1016/j.bios.2026.119049
站内全文 PDF6.2 MBPubMed 42485703杂志页 DOI
154A one-tube dual-readout biosensor for detection of nucleic acids and non-nucleic acids using CRISPR-ALP tandem assay
站内全文The Analyst · 2023 · 10.1039/d3an00918a
站内全文 PDF2.0 MBPubMed 37555739杂志页 DOI
156A PAM-Free One-Step Asymmetric RPA and CRISPR/Cas12b Combined Assay (OAR-CRISPR) for Rapid and Ultrasensitive DNA Detection
站内全文Analytical Chemistry · 2024 · 10.1021/acs.analchem.3c05545
站内全文 PDF2.9 MBPubMed 38551977杂志页 DOI
162A rapid visual detection method for Sugarcane streak mosaic virus based on one-tube RPA-CRISPR/Cas12a
站内全文Talanta · 2025 · 10.1016/j.talanta.2025.127888
站内全文 PDF4.3 MBPubMed 40049000杂志页 DOI
169A SERS-Signalled, CRISPR-Cas-Powered Bioassay for Amplification-Free and Anti-interference Detection of SARS-CoV-2 in Foods and Environmental Samples Using a Single Tube-in-Tube Vessel
站内全文Springer Protocols Handbooks · 2025 · 10.1007/978-1-0716-4358-7_3
站内全文 PDF7.8 MB↗ PubMed 按标题检索杂志页 DOI
179A Thermostable Cas12b from Brevibacillus Leverages One-pot Detection of SARS-CoV-2 Variants of Concern
站内全文EBioMedicine · 2021 · 10.1101/2021.10.15.21265066
站内全文 PDF9.5 MBPubMed 34704101杂志页 DOI
187A universal all-in-one RPA-Cas12a strategy with de novo autodesigner and its application in on-site ultrasensitive detection of DNA and RNA viruses
站内全文Biosensors and Bioelectronics · 2023 · 10.1016/j.bios.2023.115609
站内全文 PDF10.1 MBPubMed 37611446杂志页 DOI
194A general onepot-method for nucleic acid detection with CRISPR-Cas12a
站内全文OA 免费Research Square · 2020 · 10.21203/rs.3.rs-44613/v1
站内全文 PDF1011 KB出版方 OA 全文Research Square↗ PubMed 按标题检索杂志页 DOI
250Molecular Mirror Assay for Detection of SARS-CoV-2 v2
站内全文OA 免费2020 · 10.17504/protocols.io.bpdwmi7e
站内全文 PDF10.3 MB出版方 OA 全文OA↗ PubMed 按标题检索杂志页 DOI
256Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2
站内全文Proceedings of the National Academy of Sciences · 2022 · 10.1073/pnas.2118260119
站内全文 PDF2.8 MBPubMed 35763567杂志页 DOI
255Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2-SM· 补充材料
站内全文补充材料Proceedings of the National Academy of Sciences · 2022 · 10.1073/pnas.2118260119
站内全文 PDF53.5 MBPubMed 35763567杂志页 DOI
438Csm6-DNAzyme Tandem Assay for One-Pot and Sensitive Analysis of Lead Pollution and Bioaccumulation in Mice
站内全文Analytical Chemistry · 2022 · 10.1021/acs.analchem.2c04589
站内全文 PDF3.3 MBPubMed 36423327杂志页 DOI
452Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing
站内全文
站内全文 PDF625 KB
451Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing-SM· 补充材料
站内全文补充材料
站内全文 PDF5.2 MB
466Development of a Single-Tube Rpa/Crispr-Cas12a Detection Platform for Monkeypox Virus
站内全文SSRN Electronic Journal · 2024 · 10.2139/ssrn.5042002
站内全文 PDF7.7 MB↗ PubMed 按标题检索杂志页 DOI
496DNAzyme-Triggered Equilibrium Transfer with Self-Activated CRISPR-Cas12a Biosensor Enables One-Pot Diagnosis of Nucleic Acids
站内全文Analytical Chemistry · 2025 · 10.1021/acs.analchem.4c06066
站内全文 PDF6.7 MBPubMed 39889213杂志页 DOI
495DNAzyme-Triggered Equilibrium Transfer with Self-Activated CRISPR-Cas12a Biosensor Enables One-Pot Diagnosis of Nucleic Acids-SM· 补充材料
站内全文补充材料Analytical Chemistry · 2025 · 10.1021/acs.analchem.4c06066
站内全文 PDF1.0 MBPubMed 39889213杂志页 DOI
523Dynamic aqueous multiphase reaction system for simple, sensitive and quantitative one-pot CRISPR-Cas12a based molecular diagnosis
站内全文OA 免费Analytical Chemistry · 2020 · 10.1101/2020.03.21.001644
站内全文 PDF1.6 MB出版方 OA 全文Analytical ChemistryPubMed 32390420杂志页 DOI
524Dynamic bidirectional diffusion-controlled multi-enzyme system for one-pot viral detection
站内全文Journal of Advanced Research · 2026 · 10.1016/j.jare.2026.07.019
站内全文 PDF2.8 MBPubMed 42413656杂志页 DOI
547Efficient, Rapid, and Sensitive Detection of Plant RNA Viruses With One-Pot RT-RPA–CRISPR/Cas12a Assay
站内全文OA 免费Frontiers in Microbiology · 2020 · 10.3389/fmicb.2020.610872
站内全文 PDF5.6 MB出版方 OA 全文Frontiers in MicrobiologyPubMed 33391239杂志页 DOI
551Elimination of cis -cleavage in CRISPR diagnostics for one-pot rapid nucleic acid detection
站内全文OA 免费Nucleic Acids Research · 2026 · 10.1093/nar/gkag267
站内全文 PDF3.3 MB出版方 OA 全文Nucleic Acids ResearchPubMed 41885207杂志页 DOI
586Establishment of One-Pot ERA-CRISPR/Cas12a-Based Rapid Visual Assays and a TaqMan Quantitative PCR Assay for Lactococcus garvieae
站内全文Microorganisms · 2026 · 10.3390/microorganisms14040830
站内全文 PDF16.0 MBPubMed 42075227杂志页 DOI
606Fast and visual detection of nucleic acids using a one-step RPA-CRISPR detection (ORCD) system unrestricted by the PAM
站内全文Analytica Chimica Acta · 2023 · 10.1016/j.aca.2023.340938
站内全文 PDF8.7 MBPubMed 36813457杂志页 DOI
649Glycerol Additive Boosts 100-fold Sensitivity Enhancement for One-Pot RPA-CRISPR/Cas12a Assay
站内全文Analytical Chemistry · 2022 · 10.1021/acs.analchem.2c00616
站内全文 PDF3.3 MBPubMed 35635176杂志页 DOI
650Glycine/PVP-Enabled One-Pot CRISPR/Cas13a Detection of Vibrio parahaemolyticus by Tetrahedron-Mediated Electrochemistry
站内全文ACS Sensors · 2026 · 10.1021/acssensors.6c00902
站内全文 PDF2.8 MBPubMed 42215315杂志页 DOI
651Glycine/PVP-Enabled One-Pot CRISPR/Cas13a Detection of Vibrio parahaemolyticus by Tetrahedron-Mediated Electrochemistry-SM· 补充材料
站内全文补充材料ACS Sensors · 2026 · 10.1021/acssensors.6c00902
站内全文 PDF898 KBPubMed 42215315杂志页 DOI
686Identification of thermotolerant non-canonical PAMs for robust one-pot CRISPR-Cas12a detection
站内全文Nature Communications · 2026 · 10.1038/s41467-026-68476-3
站内全文 PDF20.0 MBPubMed 41545355杂志页 DOI
687Identification of thermotolerant non-canonical PAMs for robust one-pot CRISPR-Cas12a detection-SM· 补充材料
站内全文补充材料Nature Communications · 2026 · 10.1038/s41467-026-68476-3
站内全文 PDF3.8 MBPubMed 41545355杂志页 DOI
692Improved CRISPR‐Cas12a‐assisted one‐pot DNA editing method enables seamless DNA editing
站内全文Biotechnology and Bioengineering · 2019 · 10.1002/bit.26938
站内全文 PDF1.0 MBPubMed 30730047杂志页 DOI
713Integrating Reverse Transcription Recombinase Polymerase Amplification with CRISPR Technology for the One-Tube Assay of RNA
站内全文OA 免费Analytical Chemistry · 2021 · 10.1021/acs.analchem.1c03456
站内全文 PDF5.4 MB出版方 OA 全文Analytical ChemistryPubMed 34506127杂志页 DOI
721Isothermal Background-Free Nucleic Acid Quantification by a One-Pot Cas13a Assay Using Droplet Microfluidics
站内全文Analytical Chemistry · 2022 · 10.1021/acs.analchem.2c00067
站内全文 PDF4.3 MBPubMed 35387453杂志页 DOI
838One-Pot Rpa-Crispr/Cas12a Integrated Dual-Mode Electrochemical Lateral Flow Strip for Ultrasensitive and Precise Detection of Salmonella
站内全文Biosensors and Bioelectronics · 2025 · 10.2139/ssrn.5151207
站内全文 PDF5.5 MBPubMed 40378589杂志页 DOI
839One-step detection of Chlamydia psittaci using recombinase polymerase amplification and CRISPR/Cas12b
站内全文AMB Express · 2026 · 10.1186/s13568-025-01997-0
站内全文 PDF3.8 MBPubMed 41483268杂志页 DOI
840One-step high-efficiency CRISPR/Cas9-mediated genome editing in <italic>Streptomyces</italic>
站内全文Acta Biochimica et Biophysica Sinica · 2015 · 10.1093/abbs/gmv007
站内全文 PDF851 KBPubMed 25739462杂志页 DOI
841One-step integration of multiple genes into the oleaginous yeast Yarrowia lipolytica
站内全文Biotechnology Letters · 2014 · 10.1007/s10529-014-1634-y
站内全文 PDF1.5 MBPubMed 25216641杂志页 DOI
842One-Tube SARS-CoV-2 Detection Platform Based on RT-RPA and CRISPR/Cas12a
站内全文OA 免费Journal of Translational Medicine · 2020 · 10.21203/rs.3.rs-96229/v1
站内全文 PDF3.9 MB出版方 OA 全文Journal of Translational MedicinePubMed 33593370杂志页 DOI
843One-Tube SARS-CoV-2 Detection Platform Based on RT-RPA and CRISPR/Cas12a
站内全文OA 免费Journal of Translational Medicine · 2020 · 10.21203/rs.3.rs-96229/v1
站内全文 PDF363 KB出版方 OA 全文Journal of Translational MedicinePubMed 33593370杂志页 DOI
845opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection
站内全文Biosensors and Bioelectronics · 2021 · 10.1016/j.bios.2020.112766
站内全文 PDF6.5 MBPubMed 33126177杂志页 DOI
868Photoactivatable CRISPR/Cas12a Strategy for One-Pot DETECTR Molecular Diagnosis
站内全文Analytical Chemistry · 2022 · 10.1021/acs.analchem.2c01193
站内全文 PDF2.5 MBPubMed 35762828杂志页 DOI
876Plug-and-Play Photo-Initiated CRISPR–Cas12a One-Pot Nucleic Acid Detection via Universal Repeat RNA Acylation Strategy
站内全文Analytical Chemistry · 2026 · 10.1021/acs.analchem.5c05769
站内全文 PDF5.3 MBPubMed 41535129杂志页 DOI
882Portable Amplification-Free Digital Droplet CRISPR/Cas12a Platform for One-Pot Multiplexed Viruses Detection with Attomolar Sensitivity
站内全文Biosensors and Bioelectronics · 2026 · 10.2139/ssrn.6135331
站内全文 PDF5.9 MBPubMed 41955813杂志页 DOI
919Rapid and sensitive Cas12a-based one-step nucleic acid detection with ssDNA-modified crRNA
站内全文Analytica Chimica Acta · 2023 · 10.1016/j.aca.2023.341622
站内全文 PDF7.2 MBPubMed 37573099杂志页 DOI
920Rapid and sensitive detection of nucleic acids using an RAA-CRISPR/Cas12b one-pot detection assay (Rcod)
站内全文Talanta · 2024 · 10.1016/j.talanta.2023.125616
站内全文 PDF5.9 MBPubMed 38277969杂志页 DOI
924Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay
站内全文Cell Reports Methods · 2023 · 10.1016/j.crmeth.2023.100620
站内全文 PDF5.8 MBPubMed 37848032杂志页 DOI
995Sensitive and Robust One-Pot RPA-CRISPR/Cas12a Assay with Elimination of cis -Cleavage
站内全文Analytical Chemistry · 2026 · 10.1021/acs.analchem.5c06674
站内全文 PDF9.7 MBPubMed 42065492杂志页 DOI
996Sensitive and Robust One-Pot RPA-CRISPR/Cas12a Assay with Elimination of cis -Cleavage-SM· 补充材料
站内全文补充材料Analytical Chemistry · 2026 · 10.1021/acs.analchem.5c06674
站内全文 PDF2.4 MBPubMed 42065492杂志页 DOI
998Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay
站内全文OA 免费Biosensors and Bioelectronics · 2020 · 10.1101/2020.11.21.20236109
站内全文 PDF3.6 MB出版方 OA 全文Biosensors and BioelectronicsPubMed 33878591杂志页 DOI
1003Enhanced SfaTnpB enables single-base-specific, one-pot nucleic acid detection for high-sensitivity diagnostics
站内全文Nucleic Acids Research · 2026 · 10.1093/nar/gkaf1433
站内全文 PDF4.6 MBPubMed 41505099杂志页 DOI
1028SPEAR: CRISPR-mediated ultrasensitive, specific and rapid one-pot detection strategy for cancer-related SNPs
站内全文OA 免费Theranostics · 2025 · 10.7150/thno.107488
站内全文 PDF5.4 MB出版方 OA 全文TheranosticsPubMed 40093900杂志页 DOI
1032Specific and visual detection of EBOV based on a one-pot RT-RAA-CRISPR/Cas12a assay
站内全文Virologica Sinica · 2025 · 10.1016/j.virs.2025.11.011
站内全文 PDF2.8 MBPubMed 41319832杂志页 DOI
1043String-Powered Microfluidic Chip Integrating Heparin-Mediated One-Pot RT-RPA/CRISPR-Cas12a for Multiplex Detection of HFMD Viruses
站内全文Analytical Chemistry · 2026 · 10.1021/acs.analchem.6c00663
站内全文 PDF9.8 MBPubMed 42083769杂志页 DOI
1091Thermodynamically programmed one-pot CRISPR platform for point-of-care SNP genotyping
站内全文OA 免费Nature Communications · 2026 · 10.21203/rs.3.rs-8802024/v1
站内全文 PDF4.5 MB出版方 OA 全文Nature CommunicationsPubMed 42414323杂志页 DOI
1129Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection
站内全文OA 免费Nature Communications · 2025 · 10.1038/s41467-025-56516-3
站内全文 PDF1.9 MB出版方 OA 全文Nature CommunicationsPubMed 39885211杂志页 DOI
1137All-in-One Dual CRISPR-Cas12a (AIOD-CRISPR) Assay: A Case for Rapid, Ultrasensitive and Visual Detection of Novel Coronavirus SARS-CoV-2 and HIV virus
站内全文OA 免费Nature Communications · 2020 · 10.1101/2020.03.19.998724
站内全文 PDF2.2 MB出版方 OA 全文Nature CommunicationsPubMed 32511323杂志页 DOI
1148Universal crRNA Acylation Strategy for Robust Photo‐Initiated One‐Pot CRISPR–Cas12a Nucleic Acid Diagnostics
站内全文OA 免费Angewandte Chemie · 2024 · 10.1002/ange.202401486
站内全文 PDF3.2 MB出版方 OA 全文Angewandte Chemie↗ PubMed 按标题检索杂志页 DOI
1175A One-Pot Toolbox Based on Cas12a/crRNA Enables Rapid Foodborne Pathogen Detection at Attomolar Level
站内全文ACS Sensors · 2020 · 10.1021/acssensors.0c00320
站内全文 PDF4.4 MBPubMed 32337966杂志页 DOI
1206Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay
站内全文OA 免费Nature Communications · 2020 · 10.1038/s41467-020-18575-6
站内全文 PDF2.1 MB出版方 OA 全文Nature Communications↗ PubMed 按标题检索杂志页 DOI

§免扩增直接检测 33 篇

14Advances in amplification-free detection of nucleic acid: CRISPR/Cas system as a powerful tool
站内全文Analytical Biochemistry · 2022 · 10.1016/j.ab.2022.114593
站内全文 PDF7.8 MBPubMed 35157895杂志页 DOI
23Amplification-free cancer diagnosis based on inhibition of Cas12a activity by site-specific 5mC-modified cfDNA
站内全文Nucleic Acids Research · 2025 · 10.1093/nar/gkaf1383
站内全文 PDF1.7 MBPubMed 41404798杂志页 DOI
24Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives
站内全文Chemical Society Reviews · 2023 · 10.1039/d2cs00594h
站内全文 PDF4.3 MBPubMed 36533412杂志页 DOI
26Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy
站内全文OA 免费Cell · 2020 · 10.1016/j.cell.2020.12.001
站内全文 PDF2.5 MB出版方 OA 全文CellPubMed 33306959杂志页 DOI
25Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy-SM· 补充材料
站内全文补充材料OA 免费Cell · 2020 · 10.1016/j.cell.2020.12.001
站内全文 PDF82 KB出版方 OA 全文CellPubMed 33306959杂志页 DOI
27Amplification-free detection of zoonotic viruses using Cas13 and multiple CRISPR RNAs
站内全文Journal of General Virology · 2025 · 10.1099/jgv.0.002169
站内全文 PDF814 KBPubMed 40462949杂志页 DOI
29Amplification-free dual-blocking autocatalytic CRISPR-Cascade for attomolar DNA detection with low nonspecific signal
站内全文Proceedings of the National Academy of Sciences · 2026 · 10.1073/pnas.2537414123
站内全文 PDF6.1 MBPubMed 41573851杂志页 DOI
30Amplification-free miRNA detection with CRISPR/Cas12a system based on fragment complementary activation strategy
站内全文OA 免费Chemical Science · 2024 · 10.1039/d4sc05647g
站内全文 PDF1.0 MB出版方 OA 全文Chemical SciencePubMed 39449688杂志页 DOI
31Amplification-free RNA detection with CRISPR–Cas13
站内全文OA 免费Communications Biology · 2021 · 10.1038/s42003-021-02001-8
站内全文 PDF805 KB出版方 OA 全文Communications BiologyPubMed 33875803杂志页 DOI
33Amplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samples
站内全文OA 免费Proceedings of the National Academy of Sciences · 2025 · 10.1073/pnas.2420166122
站内全文 PDF5.2 MB出版方 OA 全文Proceedings of the National Academy of SciencesPubMed 40063799杂志页 DOI
32Amplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samples-SM· 补充材料
站内全文补充材料OA 免费Proceedings of the National Academy of Sciences · 2025 · 10.1073/pnas.2420166122
站内全文 PDF2.0 MB出版方 OA 全文Proceedings of the National Academy of SciencesPubMed 40063799杂志页 DOI
43An amplification-free ultra-sensitive electrochemical CRISPR/Cas biosensor for drug-resistant bacteria detection
站内全文OA 免费Chemical Science · 2021 · 10.1039/d1sc02197d
站内全文 PDF821 KB出版方 OA 全文Chemical SciencePubMed 34703560杂志页 DOI
55An Ultralocalized Cas13a Assay Enables Universal and Nucleic Acid Amplification-Free Single-Molecule RNA Diagnostics
站内全文ACS Nano · 2020 · 10.1021/acsnano.0c08165
站内全文 PDF4.9 MBPubMed 33498106杂志页 DOI
85Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis
站内全文OA 免费Communications Biology · 2022 · 10.1038/s42003-022-03433-6
站内全文 PDF1.4 MB出版方 OA 全文Communications BiologyPubMed 35614128杂志页 DOI
86Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis-SM· 补充材料
站内全文补充材料OA 免费Communications Biology · 2022 · 10.1038/s42003-022-03433-6
站内全文 PDF5.2 MB出版方 OA 全文Communications BiologyPubMed 35614128杂志页 DOI
223Cas12a-based electrochemiluminescence biosensor for target amplification-free DNA detection
站内全文Biosensors and Bioelectronics · 2021 · 10.1016/j.bios.2020.112954
站内全文 PDF3.1 MBPubMed 33412428杂志页 DOI
242Cascade CRISPR/cas enables amplification-free microRNA sensing with fM-sensitivity and single-base-specificity
站内全文Chemical Communications · 2021 · 10.1039/d0cc06412b
站内全文 PDF2.5 MBPubMed 33306075杂志页 DOI
251Amplification-Free Detection of Sars-Cov-2 and Respiratory Syncytial Virus Using CRISPR Cas13a and Graphene Field-Effect Transistors
站内全文ECS Meeting Abstracts · 2023 · 10.1149/ma2023-01131313mtgabs
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355CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis
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389CRISPR-powered electrochemical microfluidic multiplexed biosensor for target amplification-free miRNA diagnostics
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410CRISPR/Cas13a powered electrochemical microfluidic biosensor for nucleic acid amplification-free miRNA diagnostics
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455Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor
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516Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level
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596Exploring a CRISPR/Cas12a-powered impedimetric biosensor for amplification-free detection of a pathogenic bacterial DNA
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622Fundamental limits of amplification-free CRISPR-Cas12 and Cas13 diagnostics
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810Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs
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885Preamplification-Free Detection of RNA N6-Methyladenosine Modification at Single-Base Resolution Using the CRISPR Tandem Assay
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886Precise amplification-free detection of highly structured RNA with an enhanced SCas12a assay
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939Rapid RNA detection through intra-enzyme chain replacement-promoted Cas13a cascade cyclic reaction without amplification
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1013Single-Molecule Characterization of CRISPR–Cas12a for Amplification-Free Genetic Testing
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1014Single-Molecule Characterization of CRISPR–Cas12a for Amplification-Free Genetic Testing-SM· 补充材料
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1023Solid-Phase Extraction and Enhanced Amplification-Free Detection of Pathogens Integrated by Dual-Functional CRISPR-Cas12a
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1140Ultrasensitive detection of clinical pathogens through a target-amplification-free collateral-cleavage-enhancing CRISPR-CasΦ tool
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§数字与定量分析 10 篇

45An automated nucleic acid detection platform using digital microfluidics with an optimized Cas12a system
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60An ultrasensitive hybridization chain reaction-amplified CRISPR-Cas12a aptasensor for extracellular vesicle surface protein quantification
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283Concurrent Detection of Protein and miRNA at the Single Extracellular Vesicle Level Using a Digital Dual CRISPR-Cas Assay
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312CRISPR Digital Sensing: From Micronano-Collaborative Chip to Biomolecular Detection
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341CRISPR-Cas12a Coupled with Platinum Nanoreporter for Visual Quantification of SNVs on a Volumetric Bar-Chart Chip
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474Digital CRISPR-based diagnostics for quantification of Candida auris and resistance mutations
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475Digital CRISPR-based diagnostics for quantification of Candida auris and resistance mutations-SM· 补充材料
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476Digital CRISPR/Cas‐Assisted Assay for Rapid and Sensitive Detection of SARS‐CoV‐2
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518Droplet pairing-merging enabled digital RPA-CRISPR/Cas12a (DIMERIC) assay for rapid and precise quantification of Hepatitis B Virus DNA
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1205A Warm-start Digital CRISPR-based Method for the Quantitative Detection of Nucleic Acids
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§多重检测与荧光编码 53 篇

81Argonaute-integrated isothermal amplification for rapid, portable, multiplex detection of SARS-CoV-2 and influenza viruses
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118A field-deployable method for single and multiplex detection of DNA or RNA from pathogens using Cas12 and Cas13
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121A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
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197Barcoded microbial system for high-resolution object provenance
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200Bead-based approaches for increased sensitivity and multiplexing of CRISPR diagnostics
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225Cas12a/blocker DNA-based multiplex nucleic acid detection system for diagnosis of high-risk human papillomavirus infection
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274Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids
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305CRISPR/Cas12a‐Enabled Multiplex Biosensing Strategy Via an Affordable and Visual Nylon Membrane Readout
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358CRISPR-Cas12a/Cas13a Multiplex Bioassay for ctDNA and miRNA by Mass Spectrometry
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359CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica
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369CRISPR/dCas9‐Mediated Multiplex Gene Repression in Streptomyces
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375CRISPR-Cas-amplified urine biomarkers for multiplexed and portable cancer diagnostics
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376CRISPR-Cas-amplified urine biomarkers for multiplexed and portable cancer diagnostics-SM· 补充材料
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377CRISPR-Cas-amplified urine biomarkers for multiplexed and portable cancer diagnostics
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382CRISPR-Cpf1-Assisted Multiplex Genome Editing and Transcriptional Repression in Streptomyces
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444Deaminase-mediated multiplex genome editing in Escherichia coli
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461Development and preliminary assessment of a CRISPR–Cas12a-based multiplex detection of Mycobacterium tuberculosis complex
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519Dual Cas12a and multiplex crRNA CRISPR strategy ultrasensitive detection novel circRNA biomarker for the diagnosis of ovarian cancer
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544Efficient Multiplex Genome Editing in Streptomyces via Engineered CRISPR-Cas12a Systems
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658Highly multiplexed mRNA quantitation with CRISPR-Cas13
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662High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System
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704Inducible, tunable and multiplex human gene regulation using CRISPR-Cpf1-based transcription factors
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789Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and SARS-CoV-2 variants
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790Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts
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791Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs
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794Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System
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795Multiplex gene editing in rice with simplified CRISPR‐Cpf1 and CRISPR‐Cas9 systems
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796Multiplex Gene Disruption by Targeted Base Editing of Yarrowia lipolytica Genome Using Cytidine Deaminase Combined with the CRISPR/Cas9 System
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797Multiplex genome editing of microorganisms using CRISPR-Cas
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798Multiplex Genome Engineering Methods for Yeast Cell Factory Development
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821Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9
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846Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration
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946Regulating CRISPR/Cas12a trans-cleavage on the hairpin DNA–MB nanointerface for enhanced multiplexed sensing application
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1024Space-coded microchip for multiplexed respiratory virus detection via CRISPR-Cas12a and RPA
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1035A split crRNA with CRISPR-Cas12a enables highly sensitive, selective, and multiplexed detection of RNA and DNA
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1176Single-digit Salmonella detection with the naked eye using bio-barcode immunoassay coupled with recombinase polymerase amplification and a CRISPR-Cas12a system
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1177Efficient Multiplex Genome Editing in Streptomyces via Engineered CRISPR-Cas12a Systems
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1181“Sample-in, result-out” liquid biopsy chip based on immunomagnetic separation and CRISPR detection for multiplex analysis of exosomal microRNAs
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1211Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants
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§甲基化与表观修饰 10 篇

19AID/APOBEC deaminases disfavor modified cytosines implicated in DNA demethylation
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98A CRISPR-based approach for targeted DNA demethylation
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266Chemical Modification Coupled with Isothermal CRISPR-Based Assay for Sensitive Detection of DNA Hydroxymethylation
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320CRISPR technologies for detecting DNA and RNA methylation: Mechanisms, platforms, and translational opportunities
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536Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion
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554Endonuclease-Assisted PAM-free Recombinase Polymerase Amplification Coupling with CRISPR/Cas12a (E-PfRPA/Cas) for Sensitive Detection of DNA Methylation
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677HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation
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777Molecular basis for methylation-sensitive editing by Cas9
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§miRNA 与短链核酸 24 篇

36AND Logic-Gate-Based CRISPR/Cas12a Biosensing Platform for the Sensitive Colorimetric Detection of Dual miRNAs
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123A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing
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159A portable system for isothermal amplification and detection of exosomal microRNAs
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166A sensitive miRNA detection method based on a split-T7 switch modulating CRISPR/Cas12a system
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167A sensitive miRNA detection method based on a split-T7 switch modulating CRISPR/Cas12a system-SM· 补充材料
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298CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells
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427CRISPR–Cas12a with split crRNA for the direct and sensitive detection of microRNA
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482Direct microRNA detection via topologically engineered CRISPR/Cas12a cascade amplification assay
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501DNA Tile and Invading Stacking Primer-Assisted CRISPR–Cas12a Multiple Amplification System for Entropy-Driven Electrochemical Detection of MicroRNA with Tunable Sensitivity
站内全文Analytical Chemistry · 2023 · 10.1021/acs.analchem.3c02603
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503DNA triangular prism nanostructure and CRISPR/Cas12a empowered electrochemical biosensor for dual detection of alpha-fetoprotein and microRNA 122
站内全文Biosensors and Bioelectronics · 2025 · 10.1016/j.bios.2025.117697
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657Highly Effective and Low-Cost MicroRNA Detection with CRISPR-Cas9
站内全文ACS Synthetic Biology · 2018 · 10.1021/acssynbio.7b00446
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669High-sensitive and rapid electrochemical detection of miRNA-31 in saliva using Cas12a-based 3D nano-harvester with improved trans-cleavage efficiency
站内全文Talanta · 2024 · 10.1016/j.talanta.2023.125066
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711Integrating CRISPR-Cas12a with a DNA circuit as a generic sensing platform for amplified detection of microRNA
站内全文Chemical Science · 2020 · 10.1039/d0sc03084h
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743Machine learning-advanced hydrogel-based transcription-coupled positive-feedback CRISPR/Cas13a analysis for novel microRNA signatures in differential diagnosis of non-small cell lung cancer
站内全文Journal of Nanobiotechnology · 2026 · 10.1186/s12951-026-04476-1
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744Machine learning-advanced hydrogel-based transcription-coupled positive-feedback CRISPR/Cas13a analysis for novel microRNA signatures in differential diagnosis of non-small cell lung cancer-SM· 补充材料
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805Mutually Activated Dual-Exponential Amplification DNA Machine Enhances Robust CRISPR/Cas12a Feedback Propagation for Ultrasensitive miRNA Detection
站内全文Analytical Chemistry · 2025 · 10.1021/acs.analchem.5c01953
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812Near-infrared:glutathione-activated CRISPR:Cas13a sensing platform for the detection of multiple microRNAs
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860PCDetection: PolyA-CRISPR/Cas12a-based miRNA detection without PAM restriction
站内全文Biosensors and Bioelectronics · 2022 · 10.1016/j.bios.2022.114497
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915Putting Non-coding RNA on Display with CRISPR
站内全文OA 免费Molecular Cell · 2015 · 10.1016/j.molcel.2015.07.002
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945Regulating cleavage activity and enabling microRNA detection with split sgRNA in Cas12b
站内全文OA 免费Nature Communications · 2025 · 10.1038/s41467-025-61748-4
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962RNA Exosome-Regulated Long Non-Coding RNA Transcription Controls Super-Enhancer Activity
站内全文OA 免费Cell · 2015 · 10.1016/j.cell.2015.04.034
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1036Split-crRNA Transcription Based Dual-Recognition Cascade Amplification Assay for Sensitive and Accurate Postoperative Delirium Related MicroRNA Analysis
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1141Ultrasensitive Detection of microRNAs by Exponential Isothermal Amplification
站内全文Angewandte Chemie · 2010 · 10.1002/ange.201001375
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1144Ultra-sensitive biosensor detection of microRNA based on the CRISPR/Cas12a system and exonuclease-assisted target recycling signal amplification
站内全文Analytical Sciences · 2025 · 10.1007/s44211-025-00755-3
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§非核酸靶标检测 40 篇

56An ultrasensitive and point-of-care sensor for the telomerase activity detection
站内全文Analytica Chimica Acta · 2020 · 10.1016/j.aca.2020.11.037
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74Aptamer assisted CRISPR-Cas12a strategy for small molecule diagnostics
站内全文Biosensors and Bioelectronics · 2021 · 10.1016/j.bios.2021.113196
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75Aptamer-based cell-free detection system to detect target protein
站内全文Synthetic and Systems Biotechnology · 2021 · 10.1016/j.synbio.2021.07.004
站内全文 PDF2.5 MBPubMed 34466691杂志页 DOI
76Aptamer-based CRISPR/Cas12a assay for the ultrasensitive detection of extracellular vesicle proteins
站内全文OA 免费Talanta · 2021 · 10.1016/j.talanta.2020.121670
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77Aptamer-Linked CRISPR/Cas12a-Based Immunoassay
站内全文Analytical Chemistry · 2021 · 10.1021/acs.analchem.0c04687
站内全文 PDF3.0 MBPubMed 33497205杂志页 DOI
83Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing
站内全文OA 免费Nature Communications · 2017 · 10.1038/s41467-017-01875-9
站内全文 PDF1.8 MB出版方 OA 全文Nature CommunicationsPubMed 29167458杂志页 DOI
89A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus
站内全文OA 免费Nature Communications · 2019 · 10.1038/s41467-019-13649-6
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101A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules
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107A CRISPR/Cas12a-powered liquid crystal aptasensor for point-of-care detection of prostate-specific antigen for early-stage cancer diagnosis
站内全文Biosensors and Bioelectronics · 2025 · 10.1016/j.bios.2025.117651
站内全文 PDF6.4 MBPubMed 40480143杂志页 DOI
128A ligation-driven CRISPR–Cas biosensing platform for non-nucleic acid target detections
站内全文Chemical Communications · 2021 · 10.1039/d1cc02578c
站内全文 PDF2.3 MBPubMed 34179901杂志页 DOI
129A ligation-driven CRISPR–Cas biosensing platform for non-nucleic acid target detections-SM· 补充材料
站内全文补充材料Chemical Communications · 2021 · 10.1039/d1cc02578c
站内全文 PDF540 KBPubMed 34179901杂志页 DOI
140A novel colorimetric aptasensor for ultrasensitive detection of aflatoxin M1 based on the combination of CRISPR-Cas12a, rolling circle amplification and catalytic activity of gold nanoparticles
站内全文Analytica Chimica Acta · 2021 · 10.1016/j.aca.2021.338549
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190A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes
站内全文Science Bulletin · 2021 · 10.1016/j.scib.2020.09.004
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195A CRISPR-driven colorimetric code platform for highly accurate telomerase activity assay
站内全文Biosensors and Bioelectronics · 2021 · 10.1016/j.bios.2020.112749
站内全文 PDF6.2 MBPubMed 33160233杂志页 DOI
231Cas14VIDet: A visual instant method free from PAM restriction for antibiotic resistance bacteria detection
站内全文Biosensors and Bioelectronics · 2025 · 10.1016/j.bios.2024.116884
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321CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules
站内全文OA 免费Chemical Science · 2021 · 10.1039/d0sc06973f
站内全文 PDF2.9 MB出版方 OA 全文Chemical SciencePubMed 34163728杂志页 DOI
339CRISPR-Cas12a-Based Aptasensor for Sensitive and Selective ATP Detection
站内全文Springer Protocols Handbooks · 2025 · 10.1007/978-1-0716-4358-7_15
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391CRISPR/Cas12a and G-quadruplex DNAzyme-driven multimodal biosensor for visual detection of Aflatoxin B1
站内全文Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · 2023 · 10.1016/j.saa.2023.123121
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392CRISPR/Cas12a collateral cleavage activity for simple and rapid detection of protein/small molecule interaction
站内全文Biosensors and Bioelectronics · 2021 · 10.1016/j.bios.2021.113587
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405CRISPR/Cas13a Signal Amplification Linked Immunosorbent Assay for Femtomolar Protein Detection
站内全文Analytical Chemistry · 2019 · 10.1021/acs.analchem.9b04403
站内全文 PDF997 KBPubMed 31849223杂志页 DOI
404CRISPR/Cas13a Signal Amplification Linked Immunosorbent Assay for Femtomolar Protein Detection-SM· 补充材料
站内全文补充材料Analytical Chemistry · 2019 · 10.1021/acs.analchem.9b04403
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407CRISPR/Cas13a Signal Amplification Linked Immunosorbent Assay for Femtomolar Protein Detection
站内全文Analytical Chemistry · 2019 · 10.1021/acs.analchem.9b04403
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412CRISPR/Cas13-Based Platforms for a Potential Next-Generation Diagnosis of Colorectal Cancer through Exosomes Micro-RNA Detection: A Review
站内全文OA 免费Cancers · 2021 · 10.3390/cancers13184640
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494DNAzyme-regulated CRISPR/Cas12a based fluorescent biosensor for sensitive detection of alkaline phosphatase activity and inhibition
站内全文Analytica Chimica Acta · 2022 · 10.1016/j.aca.2022.340518
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590Exosomes in Precision Oncology and Beyond: From Bench to Bedside in Diagnostics and Therapeutics
站内全文Cancers · 2025 · 10.3390/cancers17060940
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600Fabrication of a Highly Sensitive Aptasensor for Potassium with a Nicking Endonuclease-Assisted Signal Amplification Strategy
站内全文Analytical Chemistry · 2011 · 10.1021/ac200058r
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619Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets
站内全文Journal of the American Chemical Society · 2019 · 10.1021/jacs.9b09211
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660Highly-efficient selection of aptamers for detecting various HPV subtypes in clinical samples
站内全文Talanta · 2024 · 10.1016/j.talanta.2023.125039
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666High-Fidelity Telomerase Activity Assay Based on Light-Triggered Nucleic Acid Separation System for the Diagnosis of Bladder Cancer
站内全文Biosensors and Bioelectronics · 2025 · 10.2139/ssrn.5096867
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769MnO2 nanosheets as oxidase mimics for colorimetric detection of alkaline phosphatase activity
站内全文Microchimica Acta · 2019 · 10.1007/s00604-019-3519-1
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823Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review
站内全文Biosensors and Bioelectronics · 2022 · 10.1016/j.bios.2022.114559
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824Novel Point-of-care dIagnostics with CRISPR and Isothermal amplification and an aptamer based detection method for a cancer marker osteopontin
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866Phosphorothioate-Modified Hairpin G-Triplex Reporter-Assisted Split CRISPR/Cas12a-Powered Biosensor for “Turn-On” Fluorescent Detection of Nucleic Acid and Non-Nucleic Acid Targets
站内全文Analytical Chemistry · 2025 · 10.1021/acs.analchem.5c00140
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874Plasma-Based Ultrasensitive Detection of Mycobacterium Tuberculosis Esat6/Cfp10 Fusion Antigen Using a Crispr-Driven Aptamer Fluorescence Testing (Craft)
站内全文Biosensors and Bioelectronics · 2025 · 10.2139/ssrn.5192109
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892Precision Control of CRISPR-Cas9 Using Small Molecules and Light
站内全文Biochemistry · 2019 · 10.1021/acs.biochem.8b01202
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922Rapid and sensitive exosome detection with CRISPR/Cas12a
站内全文Analytical and Bioanalytical Chemistry · 2020 · 10.1007/s00216-019-02211-4
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955Repurposing CRISPR/Cas to Discover SARS‐CoV‐2 Detecting and Neutralizing Aptamers
站内全文OA 免费Advanced Science · 2023 · 10.1002/advs.202300656
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1025SPARK-seq: a high-throughput platform for aptamer screening and kinetic profiling
站内全文OA 免费SCIENTIA SINICA Chimica · 2026 · 10.1360/ssc-2026-0014
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1026Spatially blocked split CRISPR-Cas12a system for ultra-sensitive and versatile small molecule activation and detection
站内全文OA 免费Nature Communications · 2025 · 10.1038/s41467-025-60265-8
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1037SPRINT: a Cas13a-based platform for detection of small molecules
站内全文OA 免费Nucleic Acids Research · 2020 · 10.1093/nar/gkaa673
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§恒温扩增（RPA/LAMP/RCA/EXPAR） 33 篇

52An Isothermal Method for Sensitive Detection of Mycobacterium tuberculosis Complex Using Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a Cis and Trans Cleavage
站内全文The Journal of Molecular Diagnostics · 2020 · 10.1016/j.jmoldx.2020.04.212
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54An RPA-CRISPR/Cas12a-based rapid and sensitive nucleic acid method for detection of Toxoplasma gondii in tissue and blood samples
站内全文Microbiology Spectrum · 2026 · 10.1128/spectrum.01550-25
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161A rapid isothermal RPA–CRISPR/Cas12a assay for detection of Rickettsia rickettsii
站内全文OA 免费Frontiers in Microbiology · 2026 · 10.3389/fmicb.2026.1823193
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340CRISPR-Cas12a based internal negative control for nonspecific products of exponential rolling circle amplification
站内全文OA 免费Nucleic Acids Research · 2020 · 10.1093/nar/gkaa017
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378CRISPR-Cas-Integrated LAMP
站内全文OA 免费Biosensors · 2022 · 10.3390/bios12111035
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393CRISPR/Cas12a combined with RPA for detection of T. gondii in mouse whole blood
站内全文OA 免费Parasites & Vectors · 2023 · 10.1186/s13071-023-05868-0
站内全文 PDF4.2 MB出版方 OA 全文Parasites & VectorsPubMed 37518013杂志页 DOI
394CRISPR/Cas12a Coupled With Recombinase Polymerase Amplification for Sensitive and Specific Detection of Aphelenchoides besseyi
站内全文Frontiers in Bioengineering and Biotechnology · 2022 · 10.3389/fbioe.2022.912959
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431crRNA array-mediated CRISPR/Cas12a coupling with dual RPA for highly sensitive detection of Streptomyces aureofaciens Tü117 from hypertension with multi-signal output
站内全文Biosensors and Bioelectronics · 2025 · 10.1016/j.bios.2025.117493
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449Detection of Mitochondrial Mutations Through Isothermal Nucleic Acid Amplification Coupled With Clustered Regularly Interspaced Short Palindromic Repeat-Associated Endonuclease Cas13a
站内全文OA 免费Frontiers in Genetics · 2021 · 10.3389/fgene.2020.622671
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462Development of an RT-LAMP−CRISPR/Cas12a assay for rapid and specific detection of Bandavirus dabieense
站内全文OA 免费Journal of Microbiology · 2025 · 10.71150/jm.2506013
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463Development of a field-deployable RPA-CRISPR/Cas12a assay for the detection of Cyclospora cayetanensis in human feces
站内全文Parasites & Vectors · 2025 · 10.1186/s13071-025-07150-x
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465Development of a Reverse Transcription Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Visual and Highly Specific Identification of Zika Virus
站内全文Journal of Medical Virology · 2026 · 10.1002/jmv.70917
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471Diagnostic efficiency of RT-LAMP integrated CRISPR-Cas technique for COVID-19: A systematic review and meta-analysis
站内全文Pathogens and Global Health · 2022 · 10.1080/20477724.2022.2035625
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481Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples
站内全文OA 免费Scientific Reports · 2021 · 10.1038/s41598-020-80352-8
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497DNA helicase and helicase-nuclease enzymes with a conserved iron-sulfur cluster
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513Double Isothermal Amplification and CRISPR-Cas12a for Sensitive Detection of Citrinin
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530Early diagnosis of rabies virus infection by RPA-CRISPR techniques in a rat model
站内全文OA 免费Archives of Virology · 2021 · 10.1007/s00705-021-04970-x
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615Fully Automated CRISPR-LAMP Platform for SARS-CoV-2 Delta and Omicron Variants
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723Iterative crRNA design and a PAM-free strategy enabled an ultra-specific RPA-CRISPR/Cas12a detection platform
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747Magnetically modified bacteriophage-triggered ATP release activated EXPAR-CRISPR/Cas14a system for visual detection of Burkholderia pseudomallei
站内全文Biosensors and Bioelectronics · 2024 · 10.1016/j.bios.2024.116334
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829CRISPR-based isothermal nucleic acid detection with microfluidics
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923Rapid and sensitive Mycoplasma detection in antibody bioprocessing via RPA-CRISPR/Cas12a
站内全文Journal of Pharmaceutical and Biomedical Analysis · 2025 · 10.1016/j.jpba.2025.116904
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927Rapid Detection of Bacillus subtilis via RPA Combined with CRISPR/Cas12a
站内全文Foods · 2026 · 10.3390/foods15081419
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937Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
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981RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification
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982RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods
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983RT-RPA-Cas12a-based assay facilitates the discrimination of SARS-CoV-2 variants of concern
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1017Single-strand RPA for rapid and sensitive detection of SARS-CoV-2 RNA
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1021Smartphone-integrated RPA-CRISPR-Cas12a Detection System with Microneedle Sampling for Point-of-Care Diagnosis of Potato Late Blight in Early Stage
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1029SPECIAL: Phosphorothioate dNTP assisted RPA equipped with CRISPR/Cas12a amplifier enables high-specific nucleic acid testing
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1068Study on the detection of prostate cancer using MIRA-CRISPR:Cas12a technology
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1207Cross Priming Amplification: Mechanism and Optimization for Isothermal DNA Amplification
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1208DNA Detection Using Recombination Proteins
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§Cas12a / Cpf1 / HOLMES 212 篇

22′- O -Methyl modified guide RNA promotes the single nucleotide polymorphism (SNP) discrimination ability of CRISPR–Cas12a systems
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7Accurate and sensitive analysis of Staphylococcus aureus through CRISPR-Cas12a based recycling signal amplification cascades for early diagnosis of skin and soft tissue infections
站内全文Journal of Microbiological Methods · 2021 · 10.1016/j.mimet.2021.106167
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12Active-site arginines differentially control Cas12a DNA cleavage and specificity
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35AND logic gate-based alternating PER-Cas12a signal amplification system for ultrasensitive detection of sEVs
站内全文Talanta · 2025 · 10.1016/j.talanta.2025.128411
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42Allosteric Probe-Initiated Wash-Free Method for Sensitive Extracellular Vesicle Detection through Dual Cycle-Assisted CRISPR-Cas12a
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48An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
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58An ultrasensitive biosensing platform for FEN1 activity detection based on target-induced primer extension to trigger the collateral cleavage of CRISPR/Cas12a
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59An ultrasensitive CRISPR/Cas12a based electrochemical biosensor for Listeria monocytogenes detection
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63An ultrasensitive, rapid and portable method for screening oseltamivir-resistant virus based on CRISPR/Cas12a combined with immunochromatographic strips
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68Application of CRISPR-Cas12a Enhanced Fluorescence Assay Coupled with Nucleic Acid Amplification for the Sensitive Detection of African Swine Fever Virus
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70Applying CRISPR-Cas12a as a Signal Amplifier to Construct Biosensors for Non-DNA Targets in Ultralow Concentrations
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78Architecture of a DNA-guided Cas12a
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82Arrest of CRISPR-Cas12a by Nonspecific Single-Stranded DNA for Biosensing
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84Assembly ofFrancisella novicidaCpf1 endonuclease in complex with guide RNA and target DNA
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92A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing
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99A CRISPR-Cas12a-Based Diagnostic Method for Japanese Encephalitis Virus Genotypes I, III, and V
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100A CRISPR-Cas12a-based platform for ultrasensitive rapid highly specific detection of Mycobacterium tuberculosis in clinical application
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105A CRISPR/Cas12a Based Universal Lateral Flow Biosensor for the Sensitive and Specific Detection of African Swine-Fever Viruses in Whole Blood
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106A CRISPR/Cas12a biosensor for portable and accessible detection of Salmonella typhimurium via multi-indicator pH millidisc colorimetry and smartphone imaging platform
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110A CRISPR/Cas12a-powered gold/nickel foam surface-enhanced Raman spectroscopy biosensor for nucleic acid specific detection in foods
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112A CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants
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114A DNA concatemer-encoded CRISPR/Cas12a fluorescence sensor for sensitive detection of Pb 2+ based on DNAzymes
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117A far-red light–inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation
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125A Label-Free CRISPR/Cas12a-G4 Biosensor Integrated with FTA Card for Detection of Foodborne Pathogens
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130A method for the quantitative detection of Cas12a ribonucleoproteins
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135A multi-functional synergistic platform of Cas12a split dsDNA activators
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134A multi-functional synergistic platform of Cas12a split dsDNA activators-SM· 补充材料
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138A novel biosensor for highly sensitive DNA damage detection using TdT and CRISPR-Cas12a
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141A novel CRISPR–Cas12a-based fluorescence anisotropy method with a high signal-to-background ratio for sensitive biosensing
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146A Novel Photosensitive Nanoprobe Combined with Crispr/Cas12a for Dual Signal Amplification Detection of Angptl2
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155A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration
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158A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a trans-activity
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171A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology
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178A Survey of Genome Editing Activity for 16 Cas12a Orthologs
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189A universal and orthogonal safety valve for CRISPR/Cas12a without chemical modification or external stimulation
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191A versatile CRISPR/Cas12a-based biosensing platform coupled with a target-protected transcription strategy
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198Bar-cas12a, a novel and rapid method for plant species authentication in case of Phyllanthus amarus Schumach. & Thonn
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206Biosensing bacterial 16S rDNA by microchip electrophoresis combined with a CRISPR system based on real-time crRNA/Cas12a formation
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208Editor’s Note: Bright fluorescent nucleic acid detection with CRISPR-Cas12a and poly(thymine) templated copper nanoparticles
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216Carrying out pseudo dual nucleic acid detection from sample to visual result in a polypropylene bag with CRISPR/Cas12a
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217Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA
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218Cas12a2 elicits abortive infection via RNA-triggered destruction of double-stranded DNA
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219Cas12aVDet: A CRISPR/Cas12a-Based Platform for Rapid and Visual Nucleic Acid Detection
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221Cas12a trans-cleavage can be modulated in vitro and is active on ssDNA, dsDNA, and RNA
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220Cas12a trans-cleavage can be modulated in vitro and is active on ssDNA, dsDNA, and RNA-SM· 补充材料
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224Cas12a-based on-site and rapid nucleic acid detection of African swine fever
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226Cas12a‐based on‐site, rapid detection of genetically modified crops
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227Cas12a‐based on‐site, rapid detection of genetically modified crops
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232Cas9, Cpf1 and C2c1/2/3―What's next?
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254Characterization of a thermostable Cas12a ortholog
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258Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs
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257Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs-SM· 补充材料
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260Characterization of Cme and Yme thermostable Cas12a orthologs
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265Chemical additives-enhanced CRISPR/Cas12a-based RNA detection
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275Combining CRISPR-Cas12a with Microsphere Array-Enhanced Fluorescence for Portable Pathogen Nucleic Acid Detection
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279Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens
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342CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks
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343CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects
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344CRISPR-Cas12a Nucleases Function With Structurally Engineered crRNAs – SynThetic trAcrRNA
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345CRISPR-Cas12a Possesses Unconventional DNase Activity that Can Be Inactivated by Synthetic Oligonucleotides
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346CRISPR-Cas12a powered multifunctional DNA nanodumbbell lock biosensor for multiple molecular detection
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347CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity
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349CRISPR-Cas12a target binding unleashes single-stranded DNase activity
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351CRISPR-Cas12a target binding unleashes single-stranded DNase activity
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352CRISPR-Cas12a trans -cleaves DNA G-quadruplexes
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353CRISPR-Cas12a-Assisted Multicolor Biosensor for Semiquantitative Point-of-Use Testing of the Nopaline Synthase Terminator in Genetically Modified Crops by Unaided Eyes
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354CRISPR-Cas12a-assisted recombineering in bacteria
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357CRISPR-Cas12a-mediated dual-enzyme cascade amplification for sensitive colorimetric detection of HPV-16 target and ATP
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360CRISPR-Cas12a–assisted recombineering in bacteria
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381Efficient and Accurate Single-Base Genome Editing in Corynebacterium Glutamicum by Target-Mismatched CRISPR/Cpf1
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384CRISPR-ENHANCE: An enhanced nucleic acid detection platform using Cas12a
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395CRISPR/Cas12a Protein Switch Powered Label-Free Electrochemical Biosensor for Sensitive Viral Protease Detection
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396CRISPR/Cas12a trans-cleavage cascading dual-template exponential amplification reaction for electrochemiluminescent detection of 17β-estradiol in milk
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397CRISPR/Cas12a-assisted visual and on-site detection of porcine circovirus type 2
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398CRISPR/Cas12a-based biosensing platform for precise and efficient screening of CRISPR/Cas9-induced biallelic mutants
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399CRISPR/Cas12a-based dual amplified biosensing system for sensitive and rapid detection of polynucleotide kinase/phosphatase
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400Crispr/Cas12a-Mediated Cyclic Signal Amplification and Electrochemical Reporting Strategy for Rapid and Accurate Sensing of Vibrio Parahaemolyticus in Aquatic Foods
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402CRISPR/Cas12a-Mediated Interfacial Cleaving of Hairpin DNA Reporter for Electrochemical Nucleic Acid Sensing
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403CRISPR/Cas12a-mediated liposome-amplified strategy for the photoelectrochemical detection of nucleic acid
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428CRISPR–Cas12a-mediated DNA clamping triggers target-strand cleavage
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432CrRNA Conformation-Engineered CRISPR-Cas12a System for Robust and Ultrasensitive Nucleic Acid Detection
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436Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA
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454Detect the SARS-CoV-2 D614G mutation using engineered Cas12a guide RNA
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467Development of Cas12a-Based Cell-Free Small-Molecule Biosensors via Allosteric Regulation of CRISPR Array Expression
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484Direct Observation of DNA Target Searching and Cleavage by CRISPR-Cas12a
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485Direct repeat region 3′ end modifications regulate Cas12a activity and expand its applications
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487Direct visualization of living bacterial genotypes using CRISPR/Cas12a-circular reporter nanoprobes
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489Novel CRISPR-Cas12a Clades Discovery Using Large Language Model
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533Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a
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549Electrochemical biosensor for detecting pathogenic bacteria based on a hybridization chain reaction and CRISPR-Cas12a
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555End-point dual specific detection of nucleic acids using CRISPR/Cas12a based portable biosensor
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557Engineered circular guide RNAs boost CRISPR/Cas12a- and CRISPR/Cas13d-based DNA and RNA editing
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558Engineered Cpf1 Enzymes with Altered PAM Specificities
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561Engineered CRISPR/Cas12a Enables Rapid SARS-CoV-2 Detection
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564Engineered FnCas12a with enhanced activity through directional evolution in human cells
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572Engineering of an adaptive tandem CRISPR/Cas12a molecular amplifier permits robust analysis of Vibrio parahaemolyticus
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585Establishment of a CRISPR-Cas12a based electrochemical detection method for Burkholderia gladioli and its subspecies cocovenenans in fresh noodles and tremella
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652Gold Nanoparticle-Assisted CRISPR-Cas12a-Based Activity Assay for Highly Sensitive Detection of Trypsin
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659Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus
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661High-efficiency detection of APE1 using a defective PAM-driven CRISPR-Cas12a self-catalytic biosensor
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670High-throughput and all-solution phase African Swine Fever Virus (ASFV) detection using CRISPR-Cas12a and fluorescence based point-of-care system
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676Hollow covalent organic framework-sheltering CRISPR/Cas12a as an in-vivo nanosensor for ATP imaging
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679Hydrazone ligation assisted DNAzyme walking nanomachine coupled with CRISPR-Cas12a for lipopolysaccharide analysis
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685Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform
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689ID-CRISPR: A CRISPR/Cas12a platform for label-free and sensitive detection of rare mutant alleles using self-interference DNA hydrogel reporter
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702Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos
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714Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a
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726Kinetic analysis and engineering of thermostable Cas12a for nucleic acid detection
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727Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics
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734Large-scale structural rearrangements unleash indiscriminate nuclease activity of CRISPR-Cas12a2
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739Light‐Start CRISPR‐Cas12a Reaction with Caged crRNA Enables Rapid and Sensitive Nucleic Acid Detection
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738Light‐Start CRISPR‐Cas12a Reaction with Caged crRNA Enables Rapid and Sensitive Nucleic Acid Detection-SM· 补充材料
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742Low-background CRISPR/Cas12a sensing system with circular CRISPR RNA for amplified fluorescent detection of antibody in human serum
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748A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics: SARS-CoV-2 DETECTR v1
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753MeCas12a, a Highly Sensitive and Specific System for COVID‐19 Detection
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754Mechanics of CRISPR-Cas12a and engineered variants on λ-DNA
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757Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a
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762Mg 2+ -dependent conformational rearrangements of CRISPR-Cas12a R-loop complex are mandatory for complete double-stranded DNA cleavage
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768Mismatch-Driven CRISPR/Cas12a Biosensing of UV-Induced DNA Lesions for Environmental Solar Exposure Surveillance
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770MnO 2 Nanozyme-Mediated CRISPR-Cas12a System for the Detection of SARS-CoV-2
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778Molecular Dynamics Reveals a DNA-Induced Dynamic Switch Triggering Activation of CRISPR-Cas12a
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779Molecular engineering of CRISPR:Cas12a- from activity enhancement to exponential signal amplification
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809Naked-Eye Detection of Grapevine Red-Blotch Viral Infection Using a Plasmonic CRISPR Cas12a Assay
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815New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease
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822Non-canonical CRISPR/Cas12a-based technology: A novel horizon for biosensing in nucleic acid detection
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825A novel prokaryotic CRISPR-Cas12a based tool for programmable transcriptional activation and repression
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833Nucleic Acid Enzyme‐Activated CRISPR‐Cas12a With Circular CRISPR RNA for Biosensing
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835Nuc domain electrostatics drive the trans cleavage activity of CRISPR-Cas12a
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836Obtaining Specific Sequence Tags for Yersinia pestis and Visually Detecting Them Using the CRISPR-Cas12a System
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859PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants
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893Pre-Folded G-Quadruplex as a Tunable Reporter to Facilitate CRISPR/Cas12a-Based Visual Nucleic Acid Diagnosis
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911Protein-inorganic hybrid flowers with a two-stage accelerated strategy for stimulated activation of CRISPR/Cas12a enhance polynucleotide kinase biosensing
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912Proximity hybridization-regulated CRISPR/Cas12a-based dual signal amplification strategy for sensitive detection of circulating tumor DNA
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925Engineered CRISPR/Cas12a Enables Rapid SARS-CoV-2 Detection
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926Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics
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933Engineered CRISPR/Cas12a Enables Rapid SARS-CoV-2 Detection
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940Engineered CRISPR/Cas12a Enables Rapid SARS-CoV-2 Detection
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950Reprogrammable Gel Electrophoresis Detection Assay Using CRISPR-Cas12a and Hybridization Chain Reaction
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1046Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1
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1064Structure-Guided design of Cas12a variants improves detection of nucleic acids
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1069Surface-confined CRISPR-Cas12a biosensor with metal-enhanced fluorescence for rapid and ultrasensitive detection of SARS-CoV-2 nucleocapsid protein
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1074Synthetic Oligonucleotides Inhibit CRISPR-Cpf1-Mediated Genome Editing
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1081Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins
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1082Targeted mutagenesis in rice using CRISPR-Cpf1 system
站内全文Journal of Genetics and Genomics · 2017 · 10.1016/j.jgg.2016.12.001
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1088Target-dependent nickase activities of the CRISPR–Cas nucleases Cpf1 and Cas9
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1089Target-independent hybridization chain reaction-fluorescence resonance energy transfer for sensitive assay of ctDNA based on Cas12a
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1095The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA
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1094The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA-SM· 补充材料
站内全文补充材料Nature · 2016 · 10.1038/nature17945
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1098The crystal structure of Cpf1 in complex with CRISPR RNA
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1118Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis
站内全文OA 免费Nature Communications · 2023 · 10.21203/rs.3.rs-2586294/v1
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1127Trans-cleavage activity of Cas12a effectors can be unleashed by both double-stranded DNA and single-stranded RNA targeting in absence of PAM
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1135Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition
站内全文OA 免费Cell Research · 2016 · 10.1038/cr.2016.88
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1147Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/13a System
站内全文OA 免费Analytical Chemistry · 2020 · 10.1021/acs.analchem.9b05597
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1149Unmodificated stepless regulation of CRISPR/Cas12a multi-performance
站内全文OA 免费Nucleic Acids Research · 2023 · 10.1093/nar/gkad748
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1151Uracil-Mediated New Photospacer-Adjacent Motif of Cas12a To Realize Visualized DNA Detection at the Single-Copy Level Free from Contamination
站内全文Analytical Chemistry · 2019 · 10.1021/acs.analchem.9b02554
站内全文 PDF1.5 MBPubMed 31403279杂志页 DOI
1194Chemically Modified Cpf1-CRISPR RNAs Mediate Efficient Genome Editing in Mammalian Cells
站内全文OA 免费Molecular Therapy · 2018 · 10.1016/j.ymthe.2018.02.031
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1204Selective endpoint visualized detection of Vibrio parahaemolyticus with CRISPR/Cas12a assisted PCR using thermal cycler for on-site application
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1214One-pot RPA-CRISPR/Cas12a assay for rapid and sensitive detection of Sclerotium rolfsii in Glycine max
站内全文OA 免费Frontiers in Plant Science · 2026 · 10.3389/fpls.2026.1927846
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1216DNA-guided CRISPR–Cas12a effectors for programmable RNA recognition and cleavage
站内全文OA 免费Nature Biotechnology · 2026 · 10.1038/s41587-026-03120-5
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§Cas12b / Cas12f / Cas14 / 其他 V 型 44 篇

8Accurate, Sensitive, and Rapid Detection of Pseudomonas aeruginosa Based on CRISPR/Cas12b with One Fluid-Handling Step
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49An engineered xCas12i with high activity, high specificity and broad PAM range
站内全文OA 免费Protein & Cell · 2022 · 10.1101/2022.06.15.496255
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147A novel thermal Cas12b from a hot spring bacterium with high target mismatch tolerance and robust DNA cleavage efficiency
站内全文International Journal of Biological Macromolecules · 2020 · 10.1016/j.ijbiomac.2020.01.079
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157A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity
站内全文Science · 2020 · 10.1126/science.abb6151
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165A scoutRNA Is Required for Some Type V CRISPR-Cas Systems
站内全文Molecular Cell · 2020 · 10.1016/j.molcel.2020.06.022
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211C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism
站内全文OA 免费Molecular Cell · 2017 · 10.1016/j.molcel.2016.11.040
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230Cas13b is a Type VI-B CRISPR-associated RNA-Guided RNase differentially regulated by accessory proteins Csx27 and Csx28
站内全文OA 免费Molecular Cell · 2016 · 10.1101/092577
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282Comprehensive assessment of miniature CRISPR-Cas12f nucleases for gene disruption
站内全文OA 免费Nature Communications · 2022 · 10.1038/s41467-022-33346-1
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413CRISPR/Cas14 provides a promising platform in facile and versatile aptasensing with improved sensitivity
站内全文Talanta · 2023 · 10.1016/j.talanta.2022.124120
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433Cryo-EM structure of the RNA-guided ribonuclease Cas12g
站内全文Nature Chemical Biology · 2021 · 10.1038/s41589-020-00721-2
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468Development of CRISPR/Cas12b-Based Multiple Cross Displacement Amplification Technique for the Detection of Mycobacterium tuberculosis Complex in Clinical Settings
站内全文OA 免费Microbiology Spectrum · 2023 · 10.1128/spectrum.03475-22
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537Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus
站内全文OA 免费Nature Biotechnology · 2021 · 10.1038/s41587-021-01009-z
站内全文 PDF2.8 MB出版方 OA 全文Nature BiotechnologyPubMed 34475560杂志页 DOI
538Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus-SM· 补充材料
站内全文补充材料OA 免费Nature Biotechnology · 2021 · 10.1038/s41587-021-01009-z
站内全文 PDF2.4 MB出版方 OA 全文Nature BiotechnologyPubMed 34475560杂志页 DOI
574Engineering of CRISPR-Cas12b for human genome editing
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站内全文 PDF1.6 MB出版方 OA 全文Nature CommunicationsPubMed 30670702杂志页 DOI
573Engineering of CRISPR-Cas12b for human genome editing-SM· 补充材料
站内全文补充材料OA 免费Nature Communications · 2019 · 10.1038/s41467-018-08224-4
站内全文 PDF8.7 MB出版方 OA 全文Nature CommunicationsPubMed 30670702杂志页 DOI
617Functionally diverse type V CRISPR-Cas systems
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616Functionally diverse type V CRISPR-Cas systems-SM· 补充材料
站内全文补充材料
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766Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells
站内全文OA 免费Nature Communications · 2021 · 10.1038/s41467-021-26469-4
站内全文 PDF1.4 MB出版方 OA 全文Nature CommunicationsPubMed 34702830杂志页 DOI
826Novel Type V-A CRISPR Effectors Are Active Nucleases with Expanded Targeting Capabilities
站内全文The CRISPR Journal · 2020 · 10.1089/crispr.2020.0043
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855PAM recognition by miniature CRISPR nucleases triggers programmable double-stranded DNA target cleavage
站内全文OA 免费bioRxiv (Cold Spring Harbor Laboratory) · 2019 · 10.1101/654897
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856PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage
站内全文OA 免费Nucleic Acids Research · 2020 · 10.1093/nar/gkaa208
站内全文 PDF2.8 MB出版方 OA 全文Nucleic Acids ResearchPubMed 32246713杂志页 DOI
857PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease
站内全文OA 免费Cell · 2016 · 10.1016/j.cell.2016.11.053
站内全文 PDF9.3 MB出版方 OA 全文CellPubMed 27984729杂志页 DOI
908Programmed DNA destruction by miniature CRISPR-Cas14 enzymes
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站内全文 PDF1.3 MBPubMed 30337455杂志页 DOI
907Programmed DNA destruction by miniature CRISPR-Cas14 enzymes-SM· 补充材料
站内全文补充材料Science · 2018 · 10.1126/science.aav4294
站内全文 PDF1.8 MBPubMed 30337455杂志页 DOI
909Programmed genome editing by a miniature CRISPR-Cas12f nuclease
站内全文Nature Chemical Biology · 2021 · 10.1038/s41589-021-00868-6
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928Rapid detection of Mycobacterium tuberculosis in sputum using CRISPR-Cas12b combined with cross-priming amplification in a single reaction
站内全文Journal of Clinical Microbiology · 2024 · 10.1128/jcm.00923-23
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954Repurposing CRISPR-Cas12b for mammalian genome engineering
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953Repurposing CRISPR-Cas12b for mammalian genome engineering-SM· 补充材料
站内全文补充材料OA 免费Cell Discovery · 2018 · 10.1038/s41421-018-0069-3
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963RNA Guide Complementarity Prevents Self-Targeting in Type VI CRISPR Systems
站内全文OA 免费Molecular Cell · 2018 · 10.1016/j.molcel.2018.07.013
站内全文 PDF21.5 MB出版方 OA 全文Molecular CellPubMed 30122537杂志页 DOI
966RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes
站内全文OA 免费Molecular Cell · 2017 · 10.1016/j.molcel.2017.04.008
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1038Stirring Up the Type V Alphabet Soup
站内全文The CRISPR Journal · 2019 · 10.1089/crispr.2019.29044.dcs
站内全文 PDF175 KBPubMed 31021231杂志页 DOI
1044Structural basis for self-cleavage prevention by tag:anti-tag pairing complementarity in type VI Cas13 CRISPR systems
站内全文OA 免费Molecular Cell · 2021 · 10.1016/j.molcel.2020.12.033
站内全文 PDF16.0 MB出版方 OA 全文Molecular CellPubMed 33472057杂志页 DOI
1049Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2
站内全文OA 免费Nature Communications · 2020 · 10.1038/s41467-020-19072-6
站内全文 PDF3.2 MB出版方 OA 全文Nature CommunicationsPubMed 33067443杂志页 DOI
1051Structural basis of stringent PAM recognition by CRISPR-C2c1 in complex with sgRNA
站内全文OA 免费Cell Research · 2017 · 10.1038/cr.2017.46
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1056Structure and engineering of the minimal type VI CRISPR-Cas13bt3
站内全文OA 免费Molecular Cell · 2022 · 10.1016/j.molcel.2022.08.001
站内全文 PDF17.7 MB出版方 OA 全文Molecular CellPubMed 36027912杂志页 DOI
1059Structure of the miniature type V-F CRISPR-Cas effector enzyme
站内全文OA 免费Molecular Cell · 2021 · 10.1016/j.molcel.2020.11.035
站内全文 PDF8.1 MB出版方 OA 全文Molecular CellPubMed 33333018杂志页 DOI
1060Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
站内全文OA 免费Nature Communications · 2021 · 10.1038/s41467-021-24707-3
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1086Targeting Plant ssDNA Viruses with Engineered Miniature CRISPR-Cas14a
站内全文Trends in Biotechnology · 2019 · 10.1016/j.tibtech.2019.03.015
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1087Target site selection and remodelling by type V CRISPR-transposon systems
站内全文Nature · 2021 · 10.1038/s41586-021-04030-z
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1117The Versatile Type V CRISPR Effectors and Their Application Prospects
站内全文OA 免费Frontiers in Cell and Developmental Biology · 2021 · 10.3389/fcell.2020.622103
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1121Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors
站内全文OA 免费Cell · 2018 · 10.1016/j.cell.2018.02.033
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1126trans Single‐Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction
站内全文Angewandte Chemie · 2021 · 10.1002/ange.202110384
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1210CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity
站内全文OA 免费Genome Biology · 2019 · 10.1186/s13059-019-1742-z
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1217CRISPR-Cas12b-assisted nucleic acid detection platform
站内全文OA 免费bioRxiv (preprint) · 2018 · 10.1101/362889
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§Cas13 / C2c2 / SHERLOCK / Csm6 69 篇

10AcrVIB1 inhibits CRISPR-Cas13b immunity by promoting unproductive crRNA binding accessible to RNase attack
站内全文Molecular Cell · 2025 · 10.1016/j.molcel.2025.01.020
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39Anti-CRISPR prediction using deep learning reveals an inhibitor of Cas13b nucleases
站内全文Molecular Cell · 2022 · 10.1016/j.molcel.2022.05.003
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108A CRISPR/Cas13a system based on a dumbbell-shaped hairpin combined with DNA-PAINT to establish the DCP-platform for highly sensitive detection of Hantaan virus RNA
站内全文Talanta · 2025 · 10.1016/j.talanta.2025.127852
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109A CRISPR/Cas13a-powered catalytic electrochemical biosensor for successive and highly sensitive RNA diagnostics
站内全文Biosensors and Bioelectronics · 2021 · 10.1016/j.bios.2021.113027
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132A Modular and Programmable Cas13d Platform for RNA Single Nucleotide Variant Detection
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133A Multifunction Lipid-Based CRISPR-Cas13a Genetic Circuit Delivery System for Bladder Cancer Gene Therapy
站内全文ACS Synthetic Biology · 2019 · 10.1021/acssynbio.9b00349
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143A Novel Miniature CRISPR-Cas13 System for SARS-CoV-2 Diagnostics
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144A Novel Miniature CRISPR-Cas13 System for SARS-CoV-2 Diagnostics-SM· 补充材料
站内全文补充材料ACS Synthetic Biology · 2021 · 10.1021/acssynbio.1c00181
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201Bead-based RNA Detection with Cas13a: Multi-scale Design and Validation
站内全文OA 免费bioRxiv (Cold Spring Harbor Laboratory) · 2026 · 10.64898/2026.07.27.739218
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209Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing
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213C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
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263Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells
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276Compact RNA editors with small Cas13 proteins
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277Compact RNA editors with small Cas13 proteins-SM· 补充材料
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281Composition and Diversity of CRISPR-Cas13a systems in the genus Leptotrichia
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361CRISPR Cas13a: A Potential Therapeutic Cure for HIV Infection in Humans
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362CRISPR-cas13 enzymology rapidly detects SARS-CoV-2 fragments in a clinical setting
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363CRISPR-Cas13 Inhibitors Block RNA Editing in Bacteria and Mammalian Cells
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364CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms
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406CRISPR/Cas13a signal amplification linked immunosorbent assay (CLISA)
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408CRISPR/Cas13a: Compensatory Target Activation Mechanism
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409CRISPR/Cas13a-Driven Catalytic Hairpin Assembly of a Quantum Dot Nanobeacon for Viral RNA Detection
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411CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells
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426CRISPR/Cas13a-mediated interfacial cleaving of hairpin RNA reporter for PEAK1 nucleic acid sensing
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439Csx28 is a membrane pore that enhances CRISPR-Cas13b–dependent antiphage defense
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440Csx28 is a membrane pore that enhances CRISPR-Cas13b–dependent antiphage defense-SM· 补充材料
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446Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time
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450Detection of SARS-CoV-2 and Its Mutated Variants via CRISPR-Cas13-Based Transcription Amplification
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459Development and clinical evaluation of a CRISPR/Cas13a-based diagnostic test to detect Mycobacterium tuberculosis in clinical specimens
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480Direct detection of SARS-CoV-2 using CRISPR-Cas13a and a mobile phone
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527Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems
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542Efficient inhibition of HIV using CRISPR/Cas13d nuclease system
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559Engineered CRISPR-Cas13a system with enhanced target RNA cleavage activity and reduced collateral activity for therapeutic applications
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565Engineered LwaCas13a with enhanced collateral activity for nucleic acid detection
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567Engineered LwaCas13a with enhanced collateral activity for nucleic acid detection-SM· 补充材料
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663High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effect
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664High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effect-SM· 补充材料
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665High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effect
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667High-intensity vector signals for detecting SARS-CoV-2 RNA using CRISPR/Cas13a couple with stabilized graphene field-effect transistor
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671High-Throughput CRISPR–Cas13 SARS-CoV-2 Test
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750MARPLE: A Proximity-Triggered CRISPR-Cas13 Platform for Ultrasensitive Antibody Detection
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774Modular split-trigger cooperative activation enables a programmable Cas13-12 cascade
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801Multiple TMA-aided CRISPR/Cas13a platform for highly sensitive detection of IL-15 to predict immunotherapeutic response in nasopharyngeal carcinoma
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807N 1-Methyladenosine detection with CRISPR-Cas13a/C2c2
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828Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13
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830Nucleic acid detection with CRISPR-Cas13a-C2c2-s
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831Nucleic acid detection with CRISPR-Cas13a:C2c2-SM· 补充材料
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848Orthogonal inducible control of Cas13 circuits enables programmable RNA regulation in mammalian cells
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879Point-of-care testing for COVID-19 using SHERLOCK diagnostics
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903Author Correction: Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes
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929Rapid detection of SARS-CoV-2 RNA in saliva via Cas13
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932Recent progress on rapid SARS-CoV-2/COVID-19 detection by CRISPR-Cas13-based platforms
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930Recent progress on rapid SARS-CoV-2/COVID-19 detection by CRISPR-Cas13-based platforms-SM· 补充材料
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965RNA structure modulates Cas13 activity and enables mismatch detection
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968CRISPR‐Cas13: Biology, Mechanism, and Applications of RNA‐Guided, RNA‐Targeting CRISPR Systems
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977RNA-mediated CRISPR-Cas13 inhibition through crRNA structural mimicry
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991Screening for functional circular RNAs using the CRISPR-Cas13 system
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1004SHERLOCK: nucleic acid detection with CRISPR nucleases
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1010Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants
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1042Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2
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1045Structural Basis for Target Discrimination and Activation by Cas13d
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1048Structural basis for the RNA-guided ribonuclease activity of CRISPR-Cas13d
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1057Structure and mechanism of the RNA dependent RNase Cas13a from Rhodobacter capsulatus
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1066Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas
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1092The Card1 nuclease provides defence during type III CRISPR immunity
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1105The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a
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1130Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities
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1131Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection
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1213Amplification-free colorimetric detection of exosomal miRNA using a CRISPR/Cas13a-activated HRP/Au/MoS2 nanobiohybrid catalyst
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§Cas9 / dCas9 / 编辑与成像 181 篇

15Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing
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17Advancing CRISPR-Based Programmable Platforms beyond Genome Editing in Mammalian Cells
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20Allele-Specific Chromosome Removal after Cas9 Cleavage in Human Embryos
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38Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing
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40Anti-CRISPR-Based and CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2
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44An anti-CRISPR from a virulent streptococcal phage inhibits Streptococcus pyogenes Cas9
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53An NgAgo tool for genome editing: did CRISPR/Cas9 just find a competitor?
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67Applications of Cas9 as an RNA‐programmed RNA‐binding protein
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69Application of Genome Editing in Pineapple Disease Resistance Breeding: CRISPR/Cas9 Strategies
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71Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli
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72Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase
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87Automated identification of sequence-tailored Cas9 proteins using massive metagenomic data
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93A catalogue of biochemically diverse CRISPR-Cas9 orthologs
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94A chemical-inducible CRISPR–Cas9 system for rapid control of genome editing
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97A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis
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103A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
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116A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus
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119Genome-wide CRISPR-Cas9 screen identifies host factors required for influenza virus replication
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160A Precise Genome Editing Method Reveals Insights into the Activity of Eukaryotic Promoters
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182A thermostable Cas9 with increased lifetime in human plasma
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183A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila
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202Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation
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204Biochemically diverse CRISPR-Cas9 orthologs
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215Candida albicans Gene Deletion with a Transient CRISPR-Cas9 System
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233Cas9 specifies functional viral targets during CRISPR–Cas adaptation
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234CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
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235Cas9-Based Tools for Targeted Genome Editing and Transcriptional Control
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236Cas9-chromatin binding information enables more accurate CRISPR off-target prediction
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238Cas9-induced immune response: A potential caution for human genome editing
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239Cas9-mediated targeting of viral RNA in eukaryotic cells
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240Cas9-triggered chain ablation of cas9 as a gene drive brake
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241Cas9 Expression and Purification Protocol v1
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243CASLFA: CRISPR/Cas9-mediated lateral flow nucleic acid assay
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248Catalytic-state structure and engineering of Streptococcus thermophilus Cas9
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249Correction: CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool
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253In Vitro Reconstitution and Crystallization of Cas9 Endonuclease Bound to a Guide RNA and a DNA Target
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261Characterizing a thermostable Cas9 for bacterial genome editing and silencing
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262Characterizing a thermostable Cas9 for bacterial genome editing and silencing-SM· 补充材料
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264Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
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267Chimeric CRISPR guides enhance Cas9 target specificity
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278Compact SchCas9 Recognizes the Simple NNGR PAM
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280Complex transcriptional modulation with orthogonal and inducible dCas9 regulators
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284Regulating CRISPR/Cas9 Function through Conditional Guide RNA Control
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286Continuous evolution of SpCas9 variants compatible with non-G PAMs
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287Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
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290Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9
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291Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins
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295CReasPy-cloning: a method for simultaneous cloning and engineering of megabase-sized genomes in yeast using the CRISPR-Cas9 system
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297CRISPRa-mediated FOXP3 gene upregulation in mammalian cells
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299CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo
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313CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering
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318Current Applications and Future Perspective of CRISPR/Cas9 in the Diagnosis and Treatment of COVID 19: A Review
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366CRISPR-Cas9 DNA Base-Editing and Prime-Editing
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367CRISPR-Cas9 technology: applications in genome engineering, development of sequence-specific antimicrobials, and future prospects
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368Targeted genome editing using CRISPR/Cas9 system in fungi
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370CRISPR/Cas9 genome editing v1
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379CRISPR/Cas-Mediated Genome Editing for Improvement of Plant Traits
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415CRISPR/Cas9 technology: towards a new generation of improved CAR-T cells for anticancer therapies
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416CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells
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418CRISPR/Cas9-Mediated Phage Resistance Is Not Impeded by the DNA Modifications of Phage T4
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424CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides Species
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430CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
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435Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA
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442Cut Site Selection by the Two Nuclease Domains of the Cas9 RNA-guided Endonuclease
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1099The delivery challenge: fulfilling the promise of therapeutic genome editing
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1106The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity
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1107The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli
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1109The Potential of the Combination of CRISPR/Cas9 and Pluripotent Stem Cells to Provide Human Organs from Chimaeric Pigs
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1125Transposon-based genome editing of industrial microorganisms: advances, challenges, and prospects
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1128Trans-nuclease activity of Cas9 activated by DNA or RNA target binding
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1146Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
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1150Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach
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1165Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells
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1168CRISPR–Cas9 defence against HIV-1
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1173WHO sticks to 2020 governance plan for human-genome editing
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1196Heavily and Fully Modified RNAs Guide Efficient SpyCas9-Mediated Genome Editing
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1212Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor
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§病毒与病原检测 80 篇

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37Bacterial origins of cyclic nucleotide-activated antiviral immune signaling
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79Argonaute proteins from human gastrointestinal bacteria catalyze DNA-guided cleavage of single- and double-stranded DNA at 37 °C
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90A nucleus-like compartment shields bacteriophage DNA from CRISPR-Cas and restriction nucleases
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127A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma
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148A nucleotide-sensing endonuclease from the Gabija bacterial defense system
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164A CRISPR-based nucleic acid detection method for severe fever with thrombocytopenia syndrome virus
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176A streamlined CRISPR-based test for tuberculosis detection directly from sputum
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177A Streamlined Point-of-Care CRISPR Test for Tuberculosis Detection Directly from Sputum
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329Open Sharing During COVID-19: CRISPR-Based Detection Tools
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423CRISPR/Cas-Based Biosensor As a New Age Detection Method for Pathogenic Bacteria
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1132Two-Dimensional Nanozyme-Catalyzed Colorimetric CRISPR Assay for the Microfluidic Detection of Monkeypox Virus
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§POCT·微流控·试纸 7 篇

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773Modular Fabrication of Microfluidic Graphene FET for Nucleic Acids Biosensing
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853Palm-sized CRISPR sensing platform for on-site Mycoplasma pneumoniae detection
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§综述、方法与背景 13 篇

16Advances in molecular genetic systems in malaria
站内全文Nature Reviews Microbiology · 2015 · 10.1038/nrmicro3450
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170A short overview of the CRISPR-Cas adaptation stage
站内全文OA 免费Canadian Journal of Microbiology · 2021 · 10.1139/cjm-2020-0212
站内全文 PDF372 KB出版方 OA 全文Canadian Journal of MicrobiologyPubMed 32559396杂志页 DOI
184A transposable element insertion is associated with an alternative life history strategy
站内全文OA 免费Nature Communications · 2019 · 10.1038/s41467-019-13596-2
站内全文 PDF2.0 MB出版方 OA 全文Nature CommunicationsPubMed 31848330杂志页 DOI
301CRISPR Assays for Disease Diagnosis: Progress to and Barriers Remaining for Clinical Applications
站内全文OA 免费Advanced Science · 2023 · 10.1002/advs.202301697
站内全文 PDF2.3 MB出版方 OA 全文Advanced SciencePubMed 37162202杂志页 DOI
334CRISPR-based nucleic acid tests-basic principles and future potential
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338CRISPR-based strategies for sample-to-answer monkeypox detection: current status and emerging opportunities
站内全文Nanotechnology · 2024 · 10.1088/1361-6528/ad892b
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553Emerging Argonaute-based nucleic acid biosensors
站内全文Trends in Biotechnology · 2022 · 10.1016/j.tibtech.2022.03.006
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588Evolutionary Classification of CRISPR‐Cas Systems
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站内全文 PDF2.4 MB↗ PubMed 按标题检索杂志页 DOI
816Amplifying CRISPR: Next-Generation Diagnostics
站内全文OA 免费The CRISPR Journal · 2023 · 10.1089/crispr.2023.0004.editorial
站内全文 PDF439 KB出版方 OA 全文The CRISPR JournalPubMed 37071671杂志页 DOI
817Next-Generation Diagnostic with CRISPR/Cas: Beyond Nucleic Acid Detection
站内全文OA 免费International Journal of Molecular Sciences · 2022 · 10.3390/ijms23116052
站内全文 PDF4.7 MB出版方 OA 全文International Journal of Molecular SciencesPubMed 35682737杂志页 DOI
818Next-generation libraries for robust RNA interference-based genome-wide screens
站内全文OA 免费Proceedings of the National Academy of Sciences · 2015 · 10.1073/pnas.1508821112
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819Next-Generation Molecular Diagnostics Development by CRISPR/Cas Tool: Rapid Detection and Surveillance of Viral Disease Outbreaks
站内全文OA 免费Frontiers in Molecular Biosciences · 2020 · 10.3389/fmolb.2020.582499
站内全文 PDF850 KB出版方 OA 全文Frontiers in Molecular BiosciencesPubMed 33425987杂志页 DOI
1018Site-directed RNA editing: recent advances and open challenges
站内全文RNA Biology · 2021 · 10.1080/15476286.2021.1983288
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§其他 CRISPR 系统与分类学 160 篇

1The host-encoded RNase E endonuclease as the crRNA maturation enzyme in a CRISPR–Cas subtype III-Bv system
站内全文Nature Microbiology · 2018 · 10.1038/s41564-017-0103-5
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5Accelerated RNA detection using tandem CRISPR nucleases
站内全文OA 免费Nature Chemical Biology · 2021 · 10.1038/s41589-021-00842-2
站内全文 PDF6.9 MB出版方 OA 全文Nature Chemical BiologyPubMed 33791736杂志页 DOI
6Accelerated RNA detection using tandem CRISPR nucleases-SM· 补充材料
站内全文补充材料OA 免费Nature Chemical Biology · 2021 · 10.1038/s41589-021-00842-2
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18Adventure of the CRISPR Coronavirus Disease 2019 Diagnostic
站内全文The CRISPR Journal · 2020 · 10.1089/crispr.2020.29093.kro
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41Anti‐CRISPRs: The natural inhibitors for CRISPR‐Cas systems
站内全文OA 免费Animal Models and Experimental Medicine · 2019 · 10.1002/ame2.12069
站内全文 PDF1013 KB出版方 OA 全文Animal Models and Experimental MedicinePubMed 31392299杂志页 DOI
47An Engineered Cas-Transposon System for Programmable and Site-Directed DNA Transpositions
站内全文OA 免费The CRISPR Journal · 2019 · 10.1089/crispr.2019.0030
站内全文 PDF1.2 MB出版方 OA 全文The CRISPR JournalPubMed 31742433杂志页 DOI
66APOBEC3A cytidine deaminase induces RNA editing in monocytes and macrophages
站内全文OA 免费Nature Communications · 2015 · 10.1038/ncomms7881
站内全文 PDF1.8 MB出版方 OA 全文Nature CommunicationsPubMed 25898173杂志页 DOI
91A Candida albicans CRISPR system permits genetic engineering of essential genes and gene families
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96A CRISPR Path to Cutting-Edge Materials
站内全文New England Journal of Medicine · 2020 · 10.1056/nejmcibr1911506
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104A CRISPR-Cas autocatalysis-driven feedback amplification network for supersensitive DNA diagnostics
站内全文OA 免费Science Advances · 2021 · 10.1126/sciadv.abc7802
站内全文 PDF2.2 MB出版方 OA 全文Science AdvancesPubMed 33571114杂志页 DOI
113A Critical Study on DNA Probes Attached to Microplate for CRISPR/Cas12 Trans-Cleavage Activity
站内全文OA 免费Biosensors · 2023 · 10.3390/bios13080824
站内全文 PDF3.3 MB出版方 OA 全文BiosensorsPubMed 37622910杂志页 DOI
120A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks
站内全文OA 免费Cell · 2015 · 10.1016/j.cell.2015.06.059
站内全文 PDF3.2 MB出版方 OA 全文CellPubMed 26189680杂志页 DOI
124A jumbo phage that forms a nucleus-like structure evades CRISPR–Cas DNA targeting but is vulnerable to type III RNA-based immunity
站内全文OA 免费Nature Microbiology · 2019 · 10.1038/s41564-019-0612-5
站内全文 PDF4.9 MB出版方 OA 全文Nature MicrobiologyPubMed 31819217杂志页 DOI
131A Model for Resource Competition in CRISPR-Mediated Gene Repression
站内全文OA 免费bioRxiv (Cold Spring Harbor Laboratory) · 2018 · 10.1101/266015
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137A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression
站内全文Molecular Cell · 2021 · 10.1101/2021.12.06.471469
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175A solid‐phase transfection platform for arrayed CRISPR screens
站内全文OA 免费Molecular Systems Biology · 2019 · 10.15252/msb.20198983
站内全文 PDF94 KB出版方 OA 全文Molecular Systems BiologyPubMed 31885201杂志页 DOI
185A type III-E CRISPR Craspase exhibiting RNase and protease activities
站内全文OA 免费Cell Research · 2022 · 10.1038/s41422-022-00739-2
站内全文 PDF327 KB出版方 OA 全文Cell ResearchPubMed 36280711杂志页 DOI
186A Type I-F Anti-CRISPR Protein Inhibits the CRISPR-Cas Surveillance Complex by ADP-Ribosylation
站内全文OA 免费Molecular Cell · 2020 · 10.1016/j.molcel.2020.09.015
站内全文 PDF4.3 MB出版方 OA 全文Molecular CellPubMed 33049228杂志页 DOI
205WGE: a CRISPR database for genome engineering
站内全文OA 免费Bioinformatics · 2015 · 10.1093/bioinformatics/btv308
站内全文 PDF547 KB出版方 OA 全文BioinformaticsPubMed 25979474杂志页 DOI
210Building the Class 2 CRISPR-Cas Arsenal
站内全文OA 免费Molecular Cell · 2017 · 10.1016/j.molcel.2017.01.024
站内全文 PDF701 KB出版方 OA 全文Molecular CellPubMed 28157502杂志页 DOI
246CasX: a new and small CRISPR gene-editing protein
站内全文OA 免费Cell Research · 2019 · 10.1038/s41422-019-0165-4
站内全文 PDF276 KB出版方 OA 全文Cell ResearchPubMed 30992542杂志页 DOI
247Cas-CLIP: a method for customizing pooled CRISPR libraries
站内全文OA 免费bioRxiv (Cold Spring Harbor Laboratory) · 2018 · 10.1101/187757
站内全文 PDF819 KB出版方 OA 全文bioRxiv (Cold Spring Harbor Laboratory)↗ PubMed 按标题检索杂志页 DOI
252Cell-cell communication in yeast using auxin biosynthesis and auxin responsive CRISPR transcription factors
站内全文OA 免费ACS Synthetic Biology · 2015 · 10.1101/020487
站内全文 PDF1.1 MB出版方 OA 全文ACS Synthetic BiologyPubMed 26102245杂志页 DOI
269CLIPON: A CRISPR‐Enabled Strategy that Turns Commercial Pregnancy Test Strips into General Point‐of‐Need Test Devices
站内全文Angewandte Chemie · 2022 · 10.1002/ange.202115907
站内全文 PDF2.4 MB↗ PubMed 按标题检索杂志页 DOI
270Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice
站内全文OA 免费Genome Biology · 2015 · 10.1186/s13059-015-0653-x
站内全文 PDF1.6 MB出版方 OA 全文Genome BiologyPubMed 25924609杂志页 DOI
288Cornerstones of CRISPR–Cas in drug discovery and therapy
站内全文Nature Reviews Drug Discovery · 2016 · 10.1038/nrd.2016.238
站内全文 PDF11.9 MBPubMed 28008168杂志页 DOI
289Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry
站内全文OA 免费Cell Research · 2019 · 10.1038/s41422-019-0151-x
站内全文 PDF2.8 MB出版方 OA 全文Cell ResearchPubMed 30814678杂志页 DOI
292Co-opting CRISPR to deliver functional RNAs
站内全文Nature Methods · 2015 · 10.1038/nmeth.3452
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293Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity
站内全文OA 免费Cell · 2015 · 10.1016/j.cell.2015.04.027
站内全文 PDF2.0 MB出版方 OA 全文CellPubMed 25959775杂志页 DOI
294Craspase is a CRISPR RNA-guided, RNA-activated protease
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296Creating CRISPR-responsive smart materials for diagnostics and programmable cargo release
站内全文OA 免费Nature Protocols · 2020 · 10.1038/s41596-020-0367-8
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300CRISPR adaptation biases explain preference for acquisition of foreign DNA
站内全文Nature · 2015 · 10.1038/nature14302
站内全文 PDF6.7 MBPubMed 25874675杂志页 DOI
302CRISPR as a Diagnostic Tool
站内全文OA 免费Biomolecules · 2021 · 10.3390/biom11081162
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303CRISPR at lightning speeds
站内全文Science · 2020 · 10.1126/science.abc3997
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306CRISPR Cas System: a Strategic Approach in Detection of Nucleic Acids
站内全文OA 免费Microbiological Research · 2024 · 10.22541/au.170664959.98802178/v1
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311CRISPR diagnostics
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315Correction: CRISPR MultiTargeter: A Web Tool to Find Common and Unique CRISPR Single Guide RNA Targets in a Set of Similar Sequences
站内全文OA 免费PLOS ONE · 2015 · 10.1371/journal.pone.0138634
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319CRISPR technologies and the search for the PAM-free nuclease
站内全文OA 免费Nature Communications · 2021 · 10.1038/s41467-020-20633-y
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322CRISPR tools help to detect illnesses
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323CRISPR tool scales up to interrogate a huge line-up of viral suspects
站内全文Nature · 2020 · 10.1038/d41586-020-01447-w
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324CRISPR, the disruptor : Nature News & Comment
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325CRISPR-based assays for low-resource settings
站内全文OA 免费Nature Reviews Bioengineering · 2023 · 10.1038/s44222-023-00026-8
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327CRISPR‐based detection of Helicobacter pylori in stool samples
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331CRISPR-based diagnostics (James Collins)
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332CRISPR-based diagnostics
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333CRISPR-based genomic tools for the manipulation of genetically intractable microorganisms
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337CRISPR-Based Molecular Diagnostic Developed for Fusion-Driven Leukemias
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371CRISPR-CasΦ from huge phages is a hypercompact genome editor
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380CRISPR-CasΦ from huge phages is a hypercompact genome editor-SM· 补充材料
站内全文补充材料OA 免费Science · 2020 · 10.1126/science.abb1400
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372CRISPR-Cas immunity in prokaryotes
站内全文Nature · 2015 · 10.1038/nature15386
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374CRISPR‐Cas targeted plasmid integration into mammalian cells via non‐homologous end joining
站内全文Biotechnology and Bioengineering · 2015 · 10.1002/bit.25629
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383CRISPR-DO for genome-wide CRISPR design and optimization
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站内全文 PDF84 KB出版方 OA 全文BioinformaticsPubMed 27402906杂志页 DOI
385CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation: Fig. 1.
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386CRISPR-mediated biocontainment
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387CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
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390CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity
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419CRISPR/Cas and Argonaute-based biosensors for nucleic acid detection
站内全文Talanta · 2025 · 10.1016/j.talanta.2025.128210
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420CRISPR/Cas brings plant biology and breeding into the fast lane
站内全文Current Opinion in Biotechnology · 2020 · 10.1016/j.copbio.2019.08.006
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421CRISPR/cas systems redefine nucleic acid detection: Principles and methods
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425CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner
站内全文OA 免费Scientific Reports · 2019 · 10.1038/s41598-019-51891-6
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441Cutting-Edge Infectious Disease Diagnostics with CRISPR
站内全文OA 免费Cell Host & Microbe · 2018 · 10.1016/j.chom.2018.05.016
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443Cyclic tetraadenylate binding induces dimerization of protein dimers to activate a CRISPR-associated PIN nuclease
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445Degradation of Reporter Molecules Imposes a Fundamental Limit of Detection on CRISPR Diagnostics
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447Design of Nucleic Acid Biosensors Based on CRISPR/Cas Systems and Reporter Split Proteins
站内全文OA 免费Moscow University Biological Sciences Bulletin · 2021 · 10.3103/s0096392521020036
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460Development and Evaluation of A CRISPR-based Diagnostic For 2019-novel Coronavirus
站内全文OA 免费PLoS Pathogens · 2020 · 10.1101/2020.02.22.20025460
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469Development of Light‐Activated CRISPR Using Guide RNAs with Photocleavable Protectors
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479Directing cellular information flow via CRISPR signal conductors
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505DNA-guided CRISPR/Cas12 for RNA targeting
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506DNA-guided CRISPR/Cas12 for RNA targeting-SM· 补充材料
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515Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases
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514Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases-SM· 补充材料
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520Dual modes of CRISPR-associated transposon homing
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531EasyCatch, a convenient, sensitive and specific CRISPR detection system for cancer gene mutations
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568Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing
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570Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds
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576Engineering Translational Activators with CRISPR-Cas System
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580Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening
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582Enzyme kinetics of CRISPR molecular diagnostics
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599Extensive mapping of an innate immune network with CRISPR
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604Fast and sensitive CRISPR detection by minimized interference of target amplification
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607FELICX: A robust nucleic acid detection method using flap endonuclease and CRISPR-Cas12
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611Flexible guide-RNA design for CRISPR applications using Protospacer Workbench
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629Gene editing at CRISPR speed
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644Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis
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646Genome-wide target specificity of CRISPR RNA-guided adenine base editors
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647Give CRISPR a Chance: the GeneSprout Initiative
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648Glass fiber-interfaced CRISPR/Cas biosensing adaptable for diverse biomarker detection
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653GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
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688Identification of potential key genes associated with termination phase of rat liver regeneration through microarray analysis
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696Improved vectors and genome-wide libraries for CRISPR screening
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695Improved vectors and genome-wide libraries for CRISPR screening-SM· 补充材料
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707Insights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24
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709Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity
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720Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using CRISPR
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728Knowledge-based discovery for designing CRISPR-CAS systems against invading mobilomes in thermophiles
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938Rapid Reverse Genetic Screening Using CRISPR in Zebrafish
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960Correction: RNA and DNA Targeting by a Reconstituted Thermus thermophilus Type III-A CRISPR-Cas System
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972RNA-guided CRISPR-Cas technologies for genome-scale investigation of disease processes
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979RNA-triggered protein cleavage and cell growth arrest by the type III-E CRISPR nuclease-protease
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994Self versus non-self discrimination during CRISPR RNA-directed immunity
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999Sequence determinants of improved CRISPR sgRNA design
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1119Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems
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1122Translation-dependent degradation of cas12 mRNA triggered by an anti-CRISPR
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683Hydrolytic endonucleolytic ribozyme (HYER) is programmable for sequence-specific DNA cleavage-SM· 补充材料
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719IS110 transposon utilizes two mechanistically distinct RNA-guided transposition pathways
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724A Point Mutation in the Ubiquitin Ligase RNF170 That Causes Autosomal Dominant Sensory Ataxia Destabilizes the Protein and Impairs Inositol 1,4,5-Trisphosphate Receptor-mediated Ca2+ Signaling
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725Interaction of the Clostridium difficile Binary Toxin CDT and Its Host Cell Receptor, Lipolysis-stimulated Lipoprotein Receptor (LSR)
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733Large-scale parallel characterization of RNA-guided nuclease activity and specificity
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736Leveraging Endogenous ADAR for Programmable Editing on RNA
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745Direct Mutagenesis of Thousands of Genomic Targets Using Microarray-Derived Oligonucleotides
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749Mapping regulatory elements
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758Megabase-scale human genome rearrangement with programmable bridge recombinases
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764Microhomology-Mediated End Joining: A Back-up Survival Mechanism or Dedicated Pathway?
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765MIMIVIRE is a defence system in mimivirus that confers resistance to virophage
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775Modulators of Hepatic Lipoprotein Metabolism Identified in a Search for Small-Molecule Inducers of Tribbles Pseudokinase 1 Expression
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820NgAgo possesses guided DNA nicking activity
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837Oncolytic adenovirus programmed by synthetic gene circuit for cancer immunotherapy
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849Orthogonal Protein-Responsive mRNA Switches for Mammalian Synthetic Biology
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851PACE-A system for the continuous directed evolution of biomolecules
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863Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system
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869Photochemical DNA Activation
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872pH-Dependent Deformations of the Energy Landscape of Avidin-like Proteins Investigated by Single Molecule Force Spectroscopy
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890Precise RNA editing by recruiting endogenous ADARs with antisense oligonucleotides
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889Precise RNA editing by recruiting endogenous ADARs with antisense oligonucleotides-SM· 补充材料
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891Precise therapeutic gene correction by a simple nuclease-induced double-stranded break
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894Programmable autonomous synthesis of single-stranded DNA
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902Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs
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916Questions about NgAgo
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944Recording transcriptional histories using Record-seq
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964SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN
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971RNA-based recognition and targeting: sowing the seeds of specificity
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980RNA Editing and Retrotransposons in Neurology
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984S00000A-1.0 RAA核酸扩增试剂盒说明书
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985SAM-VI riboswitch structure and signature for ligand discrimination
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988A male-determining factor in the mosquito Aedes aegypti
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989Mutations in a translation initiation factor identify the target of a memory-enhancing compound
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1001Sequential cancer mutations in cultured human intestinal stem cells
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1006Short prokaryotic Argonautes provide defence against incoming mobile genetic elements through NAD + depletion
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1008Short prokaryotic Argonaute systems trigger cell death upon detection of invading DNA
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1039Peptide nucleic acid-anthraquinone conjugates: strand invasion and photoinduced cleavage of duplex DNA
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1055Structural mechanism of bridge RNA-guided recombination
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1058Structure of Taq polymerase with DNA at the polymerase active site
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1065Structure-guided discovery of highly efficient cytidine deaminases with sequence-context independence
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1075Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells
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1093The Chemical Biology of Reversible Lysine Post-translational Modifications
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1104The Manipulation of RNA‐Guided Nucleic Acid Cleavage with Ninhydrin Chemistry
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1110The prenyltransferase UBIAD1 is the target of geranylgeraniol in degradation of HMG CoA reductase
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1115The ULTIMATE Reagent: A Universal Photocleavable and Clickable Reagent for the Regiospecific and Reversible End Labeling of Any Nucleic Acid
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1120Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae
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1124Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease
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1172Wearable materials with embedded synthetic biology sensors for biomolecule detection
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1215CRISPR/Cas diagnostics for Klebsiella pneumoniae: from resistance and hypervirulence markers to clinically interpretable risk reporting
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