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按原始目录分组的 89 篇文献PDF。目录结构本身就体现了知识框架：Cas9 / Cas12a / Cas12b / Cas12f / Cas13 / Csm6、一步法体系、恒温扩增、非核酸靶标检测。

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§与PCR联用的CRISPR技术 2篇

SENA — A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection

站内全文 · 与PCR联用的CRISPR技术 · EBioMedicine 2020 · PDF · 32.0KB

CRISPR detection of circulating cell-free Mycobacterium tuberculosis DNA in adults and children, including children with HIV

站内全文 · 与PCR联用的CRISPR技术 · The Lancet Microbe 2022 · PDF · 1.2MB

§一步法CRISPR诊断体系 7篇

HOLMESv2 — A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

站内全文 · 一步法CRISPR诊断体系 · PDF · 2.6MB

CDetection — CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity

站内全文 · 一步法CRISPR诊断体系 · PDF · 1.3MB

STOPCovid — Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

站内全文 · 一步法CRISPR诊断体系 · PDF · 625.0KB

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

一步法CRISPR诊断体系 · PDF · 1.8MB

A Warm-start Digital CRISPR-based Method for the Quantitative Detection of Nucleic Acids

一步法CRISPR诊断体系 · PDF · 891.6KB

One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR:Cas12a

站内全文 · 一步法CRISPR诊断体系 · PDF · 363.0KB

Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay

一步法CRISPR诊断体系 · PDF · 9.0MB

§一管法策略：时空分离与光控激活 5篇

Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics

站内全文 · 一管法策略：时空分离与光控激活 · PNAS 2022 · PDF · 69.5KB

Light-Start CRISPR-Cas12a Reaction with Caged crRNA Enables Rapid and Sensitive Nucleic Acid Detection

站内全文 · 一管法策略：时空分离与光控激活 · Angewandte Chemie 2023 · PDF · 53.5KB

Photoactivatable CRISPR/Cas12a Strategy for One-Pot DETECTR Molecular Diagnosis

站内全文 · 一管法策略：时空分离与光控激活 · Analytical Chemistry 2022 · PDF · 51.0KB

Cas12aVDet: A CRISPR/Cas12a-Based Platform for Rapid and Visual Nucleic Acid Detection

站内全文 · 一管法策略：时空分离与光控激活 · Analytical Chemistry 2019 · PDF · 43.6KB

opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-CoV-2 detection

站内全文 · 一管法策略：时空分离与光控激活 · Biosensors and Bioelectronics 2021 · PDF · 50.6KB

§与CRISPR联用的恒温扩增技术综述 2篇

Life beyond PCR- alternative target amplification technologies for the diagnosis of infectious diseases, part II

与CRISPR联用的恒温扩增技术 · PDF · 671.3KB

Toward a next-generation diagnostic tool- A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses

与CRISPR联用的恒温扩增技术 · PDF · 5.4MB

§与CRISPR联用的恒温扩增技术/EXPAR 1篇

Isothermal reactions for the amplification of oligonucleotides

与CRISPR联用的恒温扩增技术/EXPAR · PDF · 7.3MB

§与CRISPR联用的恒温扩增技术/HCR 1篇

2004-Triggered amplification by hybridization chain reaction

与CRISPR联用的恒温扩增技术/HCR · PDF · 335.5KB

§与CRISPR联用的恒温扩增技术/HDA 1篇

2004-Helicase‐dependent isothermal DNA amplification

与CRISPR联用的恒温扩增技术/HDA · PDF · 175.6KB

§与CRISPR联用的恒温扩增技术/LAMP 4篇

2000-Loop-mediated isothermal amplification of DNA

与CRISPR联用的恒温扩增技术/LAMP · PDF · 600.1KB

2012-Cross priming amplification: mechanism and optimization for isothermal DNA amplification.

与CRISPR联用的恒温扩增技术/LAMP · PDF · 1.0MB

2016-Triggered isothermal PCR by denaturation bubble-mediated strand exchange amplification

与CRISPR联用的恒温扩增技术/LAMP · PDF · 1.9MB

2020-Primer design strategy for denaturation bubble-mediated strand exchange amplification

与CRISPR联用的恒温扩增技术/LAMP · PDF · 1.2MB

§与CRISPR联用的恒温扩增技术/NASBA 1篇

1991-Nucleic acid sequence-based amplification

与CRISPR联用的恒温扩增技术/NASBA · PDF · 3.7MB

§与CRISPR联用的恒温扩增技术/RCA 1篇

1996-Rolling Circle DNA Synthesis: Small Circular Oligonucleotides as Efficient Templates for DNA Polymerases

与CRISPR联用的恒温扩增技术/RCA · PDF · 1.1MB

§与CRISPR联用的恒温扩增技术/RPA 2篇

2006-DNA Detection Using Recombination Proteins

与CRISPR联用的恒温扩增技术/RPA · PDF · 1.2MB

2024-A PAM-Free One-Step Asymmetric RPA and CRISPR:Cas12b Combined Assay (OAR-CRISPR) for Rapid and Ultrasensitive DNA Detection

与CRISPR联用的恒温扩增技术/RPA · PDF · 2.9MB

§与CRISPR联用的恒温扩增技术/SDA 1篇

1992-Isothermal in vitro amplification of DNA by a restriction enzyme:DNA polymerase system

与CRISPR联用的恒温扩增技术/SDA · PDF · 1.3MB

§二、CRISPR诊断的发展历史 4篇

Cas12aVDet: a CRISPR:Cas12a-based platform for rapid and visual nucleic acid detection

二、CRISPR诊断的发展历史 · PDF · 565.0KB

INSPECTR-Detection of viral RNAs at ambient temperature via reporter proteins produced through the target-splinted ligation of DNA probes

二、CRISPR诊断的发展历史 · PDF · 3.0MB

Structure of the miniature type V-F CRISPR-Cas effector enzyme

二、CRISPR诊断的发展历史 · PDF · 8.1MB

2021-The Versatile Type V CRISPR Effectors and Their Application Prospects

二、CRISPR诊断的发展历史 · PDF · 1.2MB

§二、CRISPR诊断的发展历史/Cas12a 10篇

2018-CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

二、CRISPR诊断的发展历史/Cas12a · PDF · 899.0KB · 站内副本

2018-CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA-SM

二、CRISPR诊断的发展历史/Cas12a · PDF · 2.4MB · 站内副本

2018-CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

二、CRISPR诊断的发展历史/Cas12a · PDF · 1.2MB · 站内副本

2018-CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity-SM

二、CRISPR诊断的发展历史/Cas12a · PDF · 4.6MB

2018-CRISPR-Cas12a-assisted nucleic acid detection

站内全文 · 二、CRISPR诊断的发展历史/Cas12a · PDF · 704.9KB

2018-CRISPR-Cas12a-assisted nucleic acid detection-SM

未入站 · 二、CRISPR诊断的发展历史/Cas12a · PDF · 1.4MB

2019-Programmable CRISPR-responsive smart materials

站内全文 · 二、CRISPR诊断的发展历史/Cas12a · PDF · 1.3MB

2019-Programmable CRISPR-responsive smart materials-SM

未入站 · 二、CRISPR诊断的发展历史/Cas12a · PDF · 2.1MB

2021-Wearable materials with embedded synthetic biology sensors for biomolecule detection

二、CRISPR诊断的发展历史/Cas12a · PDF · 15.4MB

2021-Wearable materials with embedded synthetic biology sensors for biomolecule detection-SM

站内全文 · 二、CRISPR诊断的发展历史/Cas12a · PDF · 6.3MB

§二、CRISPR诊断的发展历史/Cas12a2 2篇

2022-Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA

二、CRISPR诊断的发展历史/Cas12a2 · PDF · 5.6MB

2022-RNA targeting unleashes indiscriminate nuclease activity of CRISPR–Cas12a2

二、CRISPR诊断的发展历史/Cas12a2 · PDF · 20.1MB

§二、CRISPR诊断的发展历史/Cas12b 7篇

2018-bioRxiv-CRISPR-Cas12b-assisted nucleic acid detection platform

站内全文 · 二、CRISPR诊断的发展历史/Cas12b · PDF · 2.2MB

2019-CDetection-CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity Open Access

站内全文 · 二、CRISPR诊断的发展历史/Cas12b · PDF · 1.3MB

2019-HOLMESv2-A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation (1)

站内全文 · 二、CRISPR诊断的发展历史/Cas12b · PDF · 2.6MB

2019-HOLMESv2-A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation-SM

站内全文 · 二、CRISPR诊断的发展历史/Cas12b · PDF · 2.2MB

2020-Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

站内全文 · 二、CRISPR诊断的发展历史/Cas12b · PDF · 633.7KB

2020-Point-of-care testing for COVID-19 using SHERLOCK diagnostics

站内全文 · 二、CRISPR诊断的发展历史/Cas12b · PDF · 967.1KB

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing-SM

站内全文 · 二、CRISPR诊断的发展历史/Cas12b · PDF · 5.3MB

§二、CRISPR诊断的发展历史/Cas12f 3篇

2018-Programmed DNA destruction by miniature CRISPR Cas14 enzymes

站内全文 · 二、CRISPR诊断的发展历史/Cas12f · PDF · 1.2MB

2018-Programmed DNA destruction by miniature CRISPR-Cas14 enzymes-SM

站内全文 · 二、CRISPR诊断的发展历史/Cas12f · PDF · 1.8MB

2020-PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage

站内全文 · 二、CRISPR诊断的发展历史/Cas12f · PDF · 2.8MB

§二、CRISPR诊断的发展历史/Cas13 13篇

2016-C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector-SM

站内全文 · 二、CRISPR诊断的发展历史/Cas13 · PDF · 7.4MB

2016-C2c2 is a single-component programmable RNA-targeting CRISPR effector

站内全文 · 二、CRISPR诊断的发展历史/Cas13 · PDF · 2.7MB

2016-Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

站内全文 · 二、CRISPR诊断的发展历史/Cas13 · PDF · 5.3MB

2017-Nucleic acid detection with CRISPR-Cas13aC2c2 copy

站内全文 · 二、CRISPR诊断的发展历史/Cas13 · PDF · 1.6MB

2017-Nucleic acid detection with CRISPR-Cas13a_C2c2-SM

二、CRISPR诊断的发展历史/Cas13 · PDF · 3.2MB

2018-Field-deployable viral diagnostics using CRISPR-Cas13

二、CRISPR诊断的发展历史/Cas13 · PDF · 433.0KB

2018-Field-deployable viral diagnostics using CRISPR-Cas13-SM

二、CRISPR诊断的发展历史/Cas13 · PDF · 1.6MB

2018-Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

二、CRISPR诊断的发展历史/Cas13 · PDF · 2.9MB

2018-Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6-SM

二、CRISPR诊断的发展历史/Cas13 · PDF · 16.2MB

2020-Massively multiplexed nucleic acid detection with Cas13

二、CRISPR诊断的发展历史/Cas13 · PDF · 25.2MB

2021-Accelerated RNA detection using tandem CRISPR nucleases-corrected

二、CRISPR诊断的发展历史/Cas13 · PDF · 4.2MB

Cell-Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

二、CRISPR诊断的发展历史/Cas13 · PDF · 2.5MB

Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants

二、CRISPR诊断的发展历史/Cas13 · PDF · 9.8MB

§二、CRISPR诊断的发展历史/Cas13/Csm6 2篇

2017-A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems

二、CRISPR诊断的发展历史/Cas13/Csm6 · PDF · 1010.0KB · 站内副本

2017-Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers

二、CRISPR诊断的发展历史/Cas13/Csm6 · PDF · 5.7MB

§二、CRISPR诊断的发展历史/Cas9 19篇

2016-Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains

二、CRISPR诊断的发展历史/Cas9 · PDF · 1.6MB

2016-Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

二、CRISPR诊断的发展历史/Cas9 · PDF · 1.5MB

2017-A facile, rapid and sensitive detection of MRSA using a CRISPR-mediated DNA FISH method, antibody-like dCas9:sgRNA complex

二、CRISPR诊断的发展历史/Cas9 · PDF · 529.8KB

2018-A CRISPR-Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

二、CRISPR诊断的发展历史/Cas9 · PDF · 1.7MB

2019-Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor

二、CRISPR诊断的发展历史/Cas9 · PDF · 3.0MB

2019-FLASH: a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences

二、CRISPR诊断的发展历史/Cas9 · PDF · 487.5KB

2019-Nucleic acid cleavage with a hyperthermophilic Cas9 from an uncultured Ignavibacterium

二、CRISPR诊断的发展历史/Cas9 · PDF · 1.1MB

2020-CRISPR:Cas9 cleavage triggered ESDR for circulating tumor DNA detection based on a 3D graphene:AuPtPd nanoflower biosensor

二、CRISPR诊断的发展历史/Cas9 · PDF · 2.6MB

2020-Colorimetric Detection of SARS-CoV-2 and Drug-Resistant pH1N1 Using CRISPR:dCas9

二、CRISPR诊断的发展历史/Cas9 · PDF · 5.6MB

2020-Targeted nanopore sequencing with Cas9-guided adapter ligation

二、CRISPR诊断的发展历史/Cas9 · PDF · 1.5MB

2021-Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor

二、CRISPR诊断的发展历史/Cas9 · PDF · 2.2MB

2024-Rapid Detection of PML::RARA Fusions in Acute Promyelocytic Leukemia: CRISPR:Cas9 Nanopore Sequencing with Adaptive Sampling

二、CRISPR诊断的发展历史/Cas9 · PDF · 1.8MB

2024-Trans-nuclease activity of Cas9 activated by DNA or RNA target binding

二、CRISPR诊断的发展历史/Cas9 · PDF · 13.4MB

2025-Enhanced detection for antibiotic resistance genes in wastewater samples using a CRISPR-enriched metagenomic method

二、CRISPR诊断的发展历史/Cas9 · PDF · 3.1MB

2025-Oxford Nanopore long-read sequencing with CRISPR:Cas9-mediated target selection for accurate characterization of copy number variants in the LDLR gene

二、CRISPR诊断的发展历史/Cas9 · PDF · 3.1MB

2025-Targeted sequencing of Enterobacterales bacteria using CRISPR-Cas9 enrichment and Oxford Nanopore Technologies

二、CRISPR诊断的发展历史/Cas9 · PDF · 2.8MB

Paired design of dCas9 as a systematic platform for the detection of featured nucleic acid sequences in pathogenic strains _si_001

二、CRISPR诊断的发展历史/Cas9 · PDF · 710.2KB

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components-SM

二、CRISPR诊断的发展历史/Cas9 · PDF · 101.5KB

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

二、CRISPR诊断的发展历史/Cas9 · PDF · 4.8MB

§二、CRISPR诊断的发展历史/研究背景 7篇

Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering

二、CRISPR诊断的发展历史/研究背景 · PDF · 1.5MB

Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants

二、CRISPR诊断的发展历史/研究背景 · PDF · 2.4MB

IMPORTANT_A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

站内全文 · 二、CRISPR诊断的发展历史/研究背景 · PDF · 2.5MB

IMPORTANT_A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity-SM

站内全文 · 二、CRISPR诊断的发展历史/研究背景 · PDF · 2.2MB

PNAS-Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria copy

二、CRISPR诊断的发展历史/研究背景 · PDF · 2.9MB

The Heroes of CRISPR

二、CRISPR诊断的发展历史/研究背景 · PDF · 737.7KB

The unsung heroes of CRISPR

二、CRISPR诊断的发展历史/研究背景 · PDF · 3.3MB

§非核酸靶标检测 3篇

A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

非核酸靶标检测 · PDF · 1.1MB

Aptamer-Linked CRISPR:Cas12a-Based Immunoassay

非核酸靶标检测 · PDF · 2.9MB

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

非核酸靶标检测 · PDF · 3.9MB